Site-Specific Chemical Labeling of Long RNA Molecules

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Site-Specific Chemical Labeling of Long RNA Molecules. / Jahn, Kasper; Olsen, Eva Maria; Nielsen, Morten Muhlig; Tørring, Thomas; Mohammadzadegan, Reza; Andersen, Ebbe Sloth; Gothelf, Kurt Vesterager; Kjems, Jørgen.

I: Bioconjugate Chemistry, Bind 22, Nr. 1, 19.01.2011, s. 95-100.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Jahn, K, Olsen, EM, Nielsen, MM, Tørring, T, Mohammadzadegan, R, Andersen, ES, Gothelf, KV & Kjems, J 2011, 'Site-Specific Chemical Labeling of Long RNA Molecules', Bioconjugate Chemistry, bind 22, nr. 1, s. 95-100. https://doi.org/10.1021/bc100422k

APA

CBE

MLA

Jahn, Kasper o.a.. "Site-Specific Chemical Labeling of Long RNA Molecules". Bioconjugate Chemistry. 2011, 22(1). 95-100. https://doi.org/10.1021/bc100422k

Vancouver

Jahn K, Olsen EM, Nielsen MM, Tørring T, Mohammadzadegan R, Andersen ES o.a. Site-Specific Chemical Labeling of Long RNA Molecules. Bioconjugate Chemistry. 2011 jan 19;22(1):95-100. https://doi.org/10.1021/bc100422k

Author

Jahn, Kasper ; Olsen, Eva Maria ; Nielsen, Morten Muhlig ; Tørring, Thomas ; Mohammadzadegan, Reza ; Andersen, Ebbe Sloth ; Gothelf, Kurt Vesterager ; Kjems, Jørgen. / Site-Specific Chemical Labeling of Long RNA Molecules. I: Bioconjugate Chemistry. 2011 ; Bind 22, Nr. 1. s. 95-100.

Bibtex

@article{ce790ba0748742e1ba1c7b3dd04225e1,
title = "Site-Specific Chemical Labeling of Long RNA Molecules",
abstract = "Site-specific labeling of RNA molecules is a valuable tool for studying their structure and function. Here, we describe a new site-specific RNA labeling method, which utilizes a DNA-templated chemical reaction to attach a label at a specific internal nucleotide in an RNA molecule. The method is nonenzymatic and based on the formation of a four-way junction, where a donor strand is chemically coupled to an acceptor strand at a specific position via an activated chemical group. A disulfide bond in the linker is subsequently cleaved under mild conditions leaving a thiol group attached to the acceptor-RNA strand. The site-specific thiol-modified target RNA can then be chemically labeled with an optional group, here demonstrated by coupling of a maleimide-functionalized fluorophore. The method is rapid and allows site specific labeling of both in vitro and in vivo synthesized RNA with a broad range of functional groups.",
author = "Kasper Jahn and Olsen, {Eva Maria} and Nielsen, {Morten Muhlig} and Thomas T{\o}rring and Reza Mohammadzadegan and Andersen, {Ebbe Sloth} and Gothelf, {Kurt Vesterager} and J{\o}rgen Kjems",
year = "2011",
month = "1",
day = "19",
doi = "10.1021/bc100422k",
language = "English",
volume = "22",
pages = "95--100",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "AMER CHEMICAL SOC",
number = "1",

}

RIS

TY - JOUR

T1 - Site-Specific Chemical Labeling of Long RNA Molecules

AU - Jahn, Kasper

AU - Olsen, Eva Maria

AU - Nielsen, Morten Muhlig

AU - Tørring, Thomas

AU - Mohammadzadegan, Reza

AU - Andersen, Ebbe Sloth

AU - Gothelf, Kurt Vesterager

AU - Kjems, Jørgen

PY - 2011/1/19

Y1 - 2011/1/19

N2 - Site-specific labeling of RNA molecules is a valuable tool for studying their structure and function. Here, we describe a new site-specific RNA labeling method, which utilizes a DNA-templated chemical reaction to attach a label at a specific internal nucleotide in an RNA molecule. The method is nonenzymatic and based on the formation of a four-way junction, where a donor strand is chemically coupled to an acceptor strand at a specific position via an activated chemical group. A disulfide bond in the linker is subsequently cleaved under mild conditions leaving a thiol group attached to the acceptor-RNA strand. The site-specific thiol-modified target RNA can then be chemically labeled with an optional group, here demonstrated by coupling of a maleimide-functionalized fluorophore. The method is rapid and allows site specific labeling of both in vitro and in vivo synthesized RNA with a broad range of functional groups.

AB - Site-specific labeling of RNA molecules is a valuable tool for studying their structure and function. Here, we describe a new site-specific RNA labeling method, which utilizes a DNA-templated chemical reaction to attach a label at a specific internal nucleotide in an RNA molecule. The method is nonenzymatic and based on the formation of a four-way junction, where a donor strand is chemically coupled to an acceptor strand at a specific position via an activated chemical group. A disulfide bond in the linker is subsequently cleaved under mild conditions leaving a thiol group attached to the acceptor-RNA strand. The site-specific thiol-modified target RNA can then be chemically labeled with an optional group, here demonstrated by coupling of a maleimide-functionalized fluorophore. The method is rapid and allows site specific labeling of both in vitro and in vivo synthesized RNA with a broad range of functional groups.

U2 - 10.1021/bc100422k

DO - 10.1021/bc100422k

M3 - Journal article

C2 - 21138255

VL - 22

SP - 95

EP - 100

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 1

ER -