Aarhus University Seal / Aarhus Universitets segl

siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Molekylærbiologisk Institut
  • Interdisciplinary Nanoscience Center
  • Aarhus School of Engineering (ASE)
  • Stereologi
  • Elektronmikroskopisk Laboratorium
  • Institut for Fysik og Astronomi
  • Molekylærbiologisk Institut
  • Det Naturvidenskabelige Fakultet
The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.
OriginalsprogEngelsk
TidsskriftMolecular Therapy
Vol/bind18
Nummer11
Sider (fra-til)2018-2027
Antal sider10
ISSN1525-0016
DOI
StatusUdgivet - 2010

Se relationer på Aarhus Universitet Citationsformater

ID: 34150616