TY - JOUR
T1 - Singlet oxygen quenching by riboflavin
AU - Insinska-Rak, Malgorzata
AU - Henke, Petr
AU - Breitenbach, Thomas
AU - Ogilby, Peter Remsen
PY - 2024/1
Y1 - 2024/1
N2 - Derivatives of riboflavin, specifically flavin mononucleotide, have recently been in the spotlight as the active component in protein-encased optogenetic sensitizers to produce singlet oxygen, O
2(a
1Δ
g). The extent to which riboflavin deactivates singlet oxygen is a key aspect of these studies. Surprisingly, there is a dearth of information on the rate constant for riboflavin-mediated singlet oxygen removal. We now report that, when riboflavin is dissolved in both aqueous and methanol solutions, the rate constant for riboflavin-mediated removal of singlet oxygen cannot be greater than ∼ 2 × 10
5 s
−1 M
−1. This number is appreciably smaller than what has been reported in the literature and, as such, our results can be significant for mechanistic interpretations of photo-processes that involve flavins in oxygenated systems.
AB - Derivatives of riboflavin, specifically flavin mononucleotide, have recently been in the spotlight as the active component in protein-encased optogenetic sensitizers to produce singlet oxygen, O
2(a
1Δ
g). The extent to which riboflavin deactivates singlet oxygen is a key aspect of these studies. Surprisingly, there is a dearth of information on the rate constant for riboflavin-mediated singlet oxygen removal. We now report that, when riboflavin is dissolved in both aqueous and methanol solutions, the rate constant for riboflavin-mediated removal of singlet oxygen cannot be greater than ∼ 2 × 10
5 s
−1 M
−1. This number is appreciably smaller than what has been reported in the literature and, as such, our results can be significant for mechanistic interpretations of photo-processes that involve flavins in oxygenated systems.
KW - Flavins
KW - Optogenetic Photosensitizers
KW - Reactive Oxygen Species (ROS)
KW - Singlet Oxygen
U2 - 10.1016/j.jphotochem.2023.115108
DO - 10.1016/j.jphotochem.2023.115108
M3 - Journal article
SN - 1010-6030
VL - 446
JO - Journal of Photochemistry and Photobiology A: Chemistry
JF - Journal of Photochemistry and Photobiology A: Chemistry
M1 - 115108
ER -