Single-molecule measurements of transient biomolecular complexes through microfluidic dilution

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Single-molecule measurements of transient biomolecular complexes through microfluidic dilution. / Horrocks, Mathew H; Rajah, Luke; Jönsson, Peter; Kjærgaard, Magnus; Vendruscolo, Michele; Knowles, Tuomas P J; Klenerman, David.

I: Analytical Chemistry, Bind 85, Nr. 14, 16.07.2013, s. 6855-9.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Horrocks, MH, Rajah, L, Jönsson, P, Kjærgaard, M, Vendruscolo, M, Knowles, TPJ & Klenerman, D 2013, 'Single-molecule measurements of transient biomolecular complexes through microfluidic dilution', Analytical Chemistry, bind 85, nr. 14, s. 6855-9. https://doi.org/10.1021/ac4010875

APA

Horrocks, M. H., Rajah, L., Jönsson, P., Kjærgaard, M., Vendruscolo, M., Knowles, T. P. J., & Klenerman, D. (2013). Single-molecule measurements of transient biomolecular complexes through microfluidic dilution. Analytical Chemistry, 85(14), 6855-9. https://doi.org/10.1021/ac4010875

CBE

Horrocks MH, Rajah L, Jönsson P, Kjærgaard M, Vendruscolo M, Knowles TPJ, Klenerman D. 2013. Single-molecule measurements of transient biomolecular complexes through microfluidic dilution. Analytical Chemistry. 85(14):6855-9. https://doi.org/10.1021/ac4010875

MLA

Vancouver

Horrocks MH, Rajah L, Jönsson P, Kjærgaard M, Vendruscolo M, Knowles TPJ o.a. Single-molecule measurements of transient biomolecular complexes through microfluidic dilution. Analytical Chemistry. 2013 jul 16;85(14):6855-9. https://doi.org/10.1021/ac4010875

Author

Horrocks, Mathew H ; Rajah, Luke ; Jönsson, Peter ; Kjærgaard, Magnus ; Vendruscolo, Michele ; Knowles, Tuomas P J ; Klenerman, David. / Single-molecule measurements of transient biomolecular complexes through microfluidic dilution. I: Analytical Chemistry. 2013 ; Bind 85, Nr. 14. s. 6855-9.

Bibtex

@article{d625b364b0de432aa80769555307a815,
title = "Single-molecule measurements of transient biomolecular complexes through microfluidic dilution",
abstract = "Single-molecule confocal microscopy experiments require concentrations which are low enough to guarantee that, on average, less than one single molecule resides in the probe volume at any given time. Such concentrations are, however, significantly lower than the dissociation constants of many biological complexes which can therefore dissociate under single-molecule conditions. To address the challenge of observing weakly bound complexes in single-molecule experiments in solution, we have designed a microfluidic device that rapidly dilutes samples by up to one hundred thousand times, allowing the observation of unstable complexes before they dissociate. The device can interface with standard biochemistry laboratory experiments and generates a spatially uniform dilution that is stable over time allowing the quantification of the relative concentrations of different molecular species.",
author = "Horrocks, {Mathew H} and Luke Rajah and Peter J{\"o}nsson and Magnus Kj{\ae}rgaard and Michele Vendruscolo and Knowles, {Tuomas P J} and David Klenerman",
year = "2013",
month = jul,
day = "16",
doi = "10.1021/ac4010875",
language = "English",
volume = "85",
pages = "6855--9",
journal = "Analytical Chemistry",
issn = "0974-7419",
publisher = "Trade Science, Inc.",
number = "14",

}

RIS

TY - JOUR

T1 - Single-molecule measurements of transient biomolecular complexes through microfluidic dilution

AU - Horrocks, Mathew H

AU - Rajah, Luke

AU - Jönsson, Peter

AU - Kjærgaard, Magnus

AU - Vendruscolo, Michele

AU - Knowles, Tuomas P J

AU - Klenerman, David

PY - 2013/7/16

Y1 - 2013/7/16

N2 - Single-molecule confocal microscopy experiments require concentrations which are low enough to guarantee that, on average, less than one single molecule resides in the probe volume at any given time. Such concentrations are, however, significantly lower than the dissociation constants of many biological complexes which can therefore dissociate under single-molecule conditions. To address the challenge of observing weakly bound complexes in single-molecule experiments in solution, we have designed a microfluidic device that rapidly dilutes samples by up to one hundred thousand times, allowing the observation of unstable complexes before they dissociate. The device can interface with standard biochemistry laboratory experiments and generates a spatially uniform dilution that is stable over time allowing the quantification of the relative concentrations of different molecular species.

AB - Single-molecule confocal microscopy experiments require concentrations which are low enough to guarantee that, on average, less than one single molecule resides in the probe volume at any given time. Such concentrations are, however, significantly lower than the dissociation constants of many biological complexes which can therefore dissociate under single-molecule conditions. To address the challenge of observing weakly bound complexes in single-molecule experiments in solution, we have designed a microfluidic device that rapidly dilutes samples by up to one hundred thousand times, allowing the observation of unstable complexes before they dissociate. The device can interface with standard biochemistry laboratory experiments and generates a spatially uniform dilution that is stable over time allowing the quantification of the relative concentrations of different molecular species.

U2 - 10.1021/ac4010875

DO - 10.1021/ac4010875

M3 - Journal article

C2 - 23782428

VL - 85

SP - 6855

EP - 6859

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0974-7419

IS - 14

ER -