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Single-Cell Transcriptome Atlas of Murine Endothelial Cells

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Single-Cell Transcriptome Atlas of Murine Endothelial Cells. / Kalucka, Joanna; de Rooij, Laura P M H; Goveia, Jermaine; Rohlenova, Katerina; Dumas, Sébastien J; Meta, Elda; Conchinha, Nadine V; Taverna, Federico; Teuwen, Laure-Anne; Veys, Koen; García-Caballero, Melissa; Khan, Shawez; Geldhof, Vincent; Sokol, Liliana; Chen, Rongyuan; Treps, Lucas; Borri, Mila; de Zeeuw, Pauline; Dubois, Charlotte; Karakach, Tobias K; Falkenberg, Kim D; Parys, Magdalena; Yin, Xiangke; Vinckier, Stefan; Du, Yuxiang; Fenton, Robert A; Schoonjans, Luc; Dewerchin, Mieke; Eelen, Guy; Thienpont, Bernard; Lin, Lin; Bolund, Lars; Li, Xuri; Luo, Yonglun; Carmeliet, Peter.

I: Cell, Bind 180, Nr. 4, 02.2020, s. 764-779.e20.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Kalucka, J, de Rooij, LPMH, Goveia, J, Rohlenova, K, Dumas, SJ, Meta, E, Conchinha, NV, Taverna, F, Teuwen, L-A, Veys, K, García-Caballero, M, Khan, S, Geldhof, V, Sokol, L, Chen, R, Treps, L, Borri, M, de Zeeuw, P, Dubois, C, Karakach, TK, Falkenberg, KD, Parys, M, Yin, X, Vinckier, S, Du, Y, Fenton, RA, Schoonjans, L, Dewerchin, M, Eelen, G, Thienpont, B, Lin, L, Bolund, L, Li, X, Luo, Y & Carmeliet, P 2020, 'Single-Cell Transcriptome Atlas of Murine Endothelial Cells', Cell, bind 180, nr. 4, s. 764-779.e20. https://doi.org/10.1016/j.cell.2020.01.015

APA

Kalucka, J., de Rooij, L. P. M. H., Goveia, J., Rohlenova, K., Dumas, S. J., Meta, E., Conchinha, N. V., Taverna, F., Teuwen, L-A., Veys, K., García-Caballero, M., Khan, S., Geldhof, V., Sokol, L., Chen, R., Treps, L., Borri, M., de Zeeuw, P., Dubois, C., ... Carmeliet, P. (2020). Single-Cell Transcriptome Atlas of Murine Endothelial Cells. Cell, 180(4), 764-779.e20. https://doi.org/10.1016/j.cell.2020.01.015

CBE

Kalucka J, de Rooij LPMH, Goveia J, Rohlenova K, Dumas SJ, Meta E, Conchinha NV, Taverna F, Teuwen L-A, Veys K, García-Caballero M, Khan S, Geldhof V, Sokol L, Chen R, Treps L, Borri M, de Zeeuw P, Dubois C, Karakach TK, Falkenberg KD, Parys M, Yin X, Vinckier S, Du Y, Fenton RA, Schoonjans L, Dewerchin M, Eelen G, Thienpont B, Lin L, Bolund L, Li X, Luo Y, Carmeliet P. 2020. Single-Cell Transcriptome Atlas of Murine Endothelial Cells. Cell. 180(4):764-779.e20. https://doi.org/10.1016/j.cell.2020.01.015

MLA

Vancouver

Kalucka J, de Rooij LPMH, Goveia J, Rohlenova K, Dumas SJ, Meta E o.a. Single-Cell Transcriptome Atlas of Murine Endothelial Cells. Cell. 2020 feb;180(4):764-779.e20. https://doi.org/10.1016/j.cell.2020.01.015

Author

Kalucka, Joanna ; de Rooij, Laura P M H ; Goveia, Jermaine ; Rohlenova, Katerina ; Dumas, Sébastien J ; Meta, Elda ; Conchinha, Nadine V ; Taverna, Federico ; Teuwen, Laure-Anne ; Veys, Koen ; García-Caballero, Melissa ; Khan, Shawez ; Geldhof, Vincent ; Sokol, Liliana ; Chen, Rongyuan ; Treps, Lucas ; Borri, Mila ; de Zeeuw, Pauline ; Dubois, Charlotte ; Karakach, Tobias K ; Falkenberg, Kim D ; Parys, Magdalena ; Yin, Xiangke ; Vinckier, Stefan ; Du, Yuxiang ; Fenton, Robert A ; Schoonjans, Luc ; Dewerchin, Mieke ; Eelen, Guy ; Thienpont, Bernard ; Lin, Lin ; Bolund, Lars ; Li, Xuri ; Luo, Yonglun ; Carmeliet, Peter. / Single-Cell Transcriptome Atlas of Murine Endothelial Cells. I: Cell. 2020 ; Bind 180, Nr. 4. s. 764-779.e20.

Bibtex

@article{8ba10bd415a240dbb628b70792e863e5,
title = "Single-Cell Transcriptome Atlas of Murine Endothelial Cells",
abstract = "The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7+ intestinal capillaries and angiogenic ECs in healthy tissues. ECs from brain/testis, liver/spleen, small intestine/colon, and skeletal muscle/heart pairwise expressed partially overlapping marker genes. Arterial, venous, and lymphatic ECs shared more markers in more tissues than did heterogeneous capillary ECs. ECs from different vascular beds (arteries, capillaries, veins, lymphatics) exhibited transcriptome similarity across tissues, but the tissue (rather than the vessel) type contributed to the EC heterogeneity. Metabolic transcriptome analysis revealed a similar tissue-grouping phenomenon of ECs and heterogeneous metabolic gene signatures in ECs between tissues and between vascular beds within a single tissue in a tissue-type-dependent pattern. The EC atlas taxonomy enabled identification of EC subclusters in public scRNA-seq datasets and provides a powerful discovery tool and resource value.",
keywords = "endothelial metabolism, endothelial-cell heterogeneity, mouse endothelial atlas, single-cell RNA-seq",
author = "Joanna Kalucka and {de Rooij}, {Laura P M H} and Jermaine Goveia and Katerina Rohlenova and Dumas, {S{\'e}bastien J} and Elda Meta and Conchinha, {Nadine V} and Federico Taverna and Laure-Anne Teuwen and Koen Veys and Melissa Garc{\'i}a-Caballero and Shawez Khan and Vincent Geldhof and Liliana Sokol and Rongyuan Chen and Lucas Treps and Mila Borri and {de Zeeuw}, Pauline and Charlotte Dubois and Karakach, {Tobias K} and Falkenberg, {Kim D} and Magdalena Parys and Xiangke Yin and Stefan Vinckier and Yuxiang Du and Fenton, {Robert A} and Luc Schoonjans and Mieke Dewerchin and Guy Eelen and Bernard Thienpont and Lin Lin and Lars Bolund and Xuri Li and Yonglun Luo and Peter Carmeliet",
note = "Copyright {\textcopyright} 2020 Elsevier Inc. All rights reserved.",
year = "2020",
month = feb,
doi = "10.1016/j.cell.2020.01.015",
language = "English",
volume = "180",
pages = "764--779.e20",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Single-Cell Transcriptome Atlas of Murine Endothelial Cells

AU - Kalucka, Joanna

AU - de Rooij, Laura P M H

AU - Goveia, Jermaine

AU - Rohlenova, Katerina

AU - Dumas, Sébastien J

AU - Meta, Elda

AU - Conchinha, Nadine V

AU - Taverna, Federico

AU - Teuwen, Laure-Anne

AU - Veys, Koen

AU - García-Caballero, Melissa

AU - Khan, Shawez

AU - Geldhof, Vincent

AU - Sokol, Liliana

AU - Chen, Rongyuan

AU - Treps, Lucas

AU - Borri, Mila

AU - de Zeeuw, Pauline

AU - Dubois, Charlotte

AU - Karakach, Tobias K

AU - Falkenberg, Kim D

AU - Parys, Magdalena

AU - Yin, Xiangke

AU - Vinckier, Stefan

AU - Du, Yuxiang

AU - Fenton, Robert A

AU - Schoonjans, Luc

AU - Dewerchin, Mieke

AU - Eelen, Guy

AU - Thienpont, Bernard

AU - Lin, Lin

AU - Bolund, Lars

AU - Li, Xuri

AU - Luo, Yonglun

AU - Carmeliet, Peter

N1 - Copyright © 2020 Elsevier Inc. All rights reserved.

PY - 2020/2

Y1 - 2020/2

N2 - The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7+ intestinal capillaries and angiogenic ECs in healthy tissues. ECs from brain/testis, liver/spleen, small intestine/colon, and skeletal muscle/heart pairwise expressed partially overlapping marker genes. Arterial, venous, and lymphatic ECs shared more markers in more tissues than did heterogeneous capillary ECs. ECs from different vascular beds (arteries, capillaries, veins, lymphatics) exhibited transcriptome similarity across tissues, but the tissue (rather than the vessel) type contributed to the EC heterogeneity. Metabolic transcriptome analysis revealed a similar tissue-grouping phenomenon of ECs and heterogeneous metabolic gene signatures in ECs between tissues and between vascular beds within a single tissue in a tissue-type-dependent pattern. The EC atlas taxonomy enabled identification of EC subclusters in public scRNA-seq datasets and provides a powerful discovery tool and resource value.

AB - The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7+ intestinal capillaries and angiogenic ECs in healthy tissues. ECs from brain/testis, liver/spleen, small intestine/colon, and skeletal muscle/heart pairwise expressed partially overlapping marker genes. Arterial, venous, and lymphatic ECs shared more markers in more tissues than did heterogeneous capillary ECs. ECs from different vascular beds (arteries, capillaries, veins, lymphatics) exhibited transcriptome similarity across tissues, but the tissue (rather than the vessel) type contributed to the EC heterogeneity. Metabolic transcriptome analysis revealed a similar tissue-grouping phenomenon of ECs and heterogeneous metabolic gene signatures in ECs between tissues and between vascular beds within a single tissue in a tissue-type-dependent pattern. The EC atlas taxonomy enabled identification of EC subclusters in public scRNA-seq datasets and provides a powerful discovery tool and resource value.

KW - endothelial metabolism

KW - endothelial-cell heterogeneity

KW - mouse endothelial atlas

KW - single-cell RNA-seq

UR - http://www.scopus.com/inward/record.url?scp=85079395140&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2020.01.015

DO - 10.1016/j.cell.2020.01.015

M3 - Journal article

C2 - 32059779

VL - 180

SP - 764-779.e20

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -