Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment
AU - Petro, Caitlin
AU - Jochum, Lara M.
AU - Schreiber, Lars
AU - Marshall, Ian P.G.
AU - Schramm, Andreas
AU - Kjeldsen, Kasper U.
PY - 2019/8
Y1 - 2019/8
N2 - The SEEP-SRB1 is a diverse clade of sulfate reducing bacteria affiliated with the deltaproteobacterial family Desulfobacteraceae. Here we report the draft genome sequences of two single cells belonging to the SEEP-SRB1 clade, isolated from marine sediment in Aarhus Bay, Denmark, by fluorescence activated cell sorting (FACS). The draft genomes measure 2.12 Mb and 0.46 Mb, with GC contents of 45% and 42%. Phylogenetic placement of the 16S rRNA genes places the SAGs within the subgroups SEEP-SRB1e and SEEP-SRB1d. While these subgroups are thought to play a role in non-methane hydrocarbon degradation, there is little genomic information available to confirm their metabolic function. As such, these novel single-cell amplified genomes provide valuable insight into the metabolic potential of this prevalent subseafloor clade.
AB - The SEEP-SRB1 is a diverse clade of sulfate reducing bacteria affiliated with the deltaproteobacterial family Desulfobacteraceae. Here we report the draft genome sequences of two single cells belonging to the SEEP-SRB1 clade, isolated from marine sediment in Aarhus Bay, Denmark, by fluorescence activated cell sorting (FACS). The draft genomes measure 2.12 Mb and 0.46 Mb, with GC contents of 45% and 42%. Phylogenetic placement of the 16S rRNA genes places the SAGs within the subgroups SEEP-SRB1e and SEEP-SRB1d. While these subgroups are thought to play a role in non-methane hydrocarbon degradation, there is little genomic information available to confirm their metabolic function. As such, these novel single-cell amplified genomes provide valuable insight into the metabolic potential of this prevalent subseafloor clade.
KW - Hydrocarbon degradation
KW - SAG
KW - SEEP-SRB
KW - Single-cell genomics
KW - Sulfate-reducing bacteria
KW - ARCHAEA
KW - ANAEROBIC OXIDATION
KW - PHYLOGENETIC ANALYSIS
KW - REDUCTASE
KW - DIVERSITY
KW - SULFATE-REDUCING BACTERIA
KW - TOOL
UR - http://www.scopus.com/inward/record.url?scp=85060434618&partnerID=8YFLogxK
U2 - 10.1016/j.margen.2019.01.004
DO - 10.1016/j.margen.2019.01.004
M3 - Journal article
AN - SCOPUS:85060434618
VL - 46
SP - 66
EP - 69
JO - Marine Genomics
JF - Marine Genomics
SN - 1874-7787
ER -