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Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment

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Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment. / Petro, Caitlin; Jochum, Lara M.; Schreiber, Lars et al.
I: Marine Genomics, Bind 46, 08.2019, s. 66-69.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Petro, Caitlin ; Jochum, Lara M. ; Schreiber, Lars et al. / Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment. I: Marine Genomics. 2019 ; Bind 46. s. 66-69.

Bibtex

@article{af6be87c090845b598300c22ec44c805,
title = "Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment",
abstract = "The SEEP-SRB1 is a diverse clade of sulfate reducing bacteria affiliated with the deltaproteobacterial family Desulfobacteraceae. Here we report the draft genome sequences of two single cells belonging to the SEEP-SRB1 clade, isolated from marine sediment in Aarhus Bay, Denmark, by fluorescence activated cell sorting (FACS). The draft genomes measure 2.12 Mb and 0.46 Mb, with GC contents of 45% and 42%. Phylogenetic placement of the 16S rRNA genes places the SAGs within the subgroups SEEP-SRB1e and SEEP-SRB1d. While these subgroups are thought to play a role in non-methane hydrocarbon degradation, there is little genomic information available to confirm their metabolic function. As such, these novel single-cell amplified genomes provide valuable insight into the metabolic potential of this prevalent subseafloor clade.",
keywords = "Hydrocarbon degradation, SAG, SEEP-SRB, Single-cell genomics, Sulfate-reducing bacteria, ARCHAEA, ANAEROBIC OXIDATION, PHYLOGENETIC ANALYSIS, REDUCTASE, DIVERSITY, SULFATE-REDUCING BACTERIA, TOOL",
author = "Caitlin Petro and Jochum, {Lara M.} and Lars Schreiber and Marshall, {Ian P.G.} and Andreas Schramm and Kjeldsen, {Kasper U.}",
year = "2019",
month = aug,
doi = "10.1016/j.margen.2019.01.004",
language = "English",
volume = "46",
pages = "66--69",
journal = "Marine Genomics",
issn = "1874-7787",
publisher = "Elsevier Science Publishers B. V.",

}

RIS

TY - JOUR

T1 - Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment

AU - Petro, Caitlin

AU - Jochum, Lara M.

AU - Schreiber, Lars

AU - Marshall, Ian P.G.

AU - Schramm, Andreas

AU - Kjeldsen, Kasper U.

PY - 2019/8

Y1 - 2019/8

N2 - The SEEP-SRB1 is a diverse clade of sulfate reducing bacteria affiliated with the deltaproteobacterial family Desulfobacteraceae. Here we report the draft genome sequences of two single cells belonging to the SEEP-SRB1 clade, isolated from marine sediment in Aarhus Bay, Denmark, by fluorescence activated cell sorting (FACS). The draft genomes measure 2.12 Mb and 0.46 Mb, with GC contents of 45% and 42%. Phylogenetic placement of the 16S rRNA genes places the SAGs within the subgroups SEEP-SRB1e and SEEP-SRB1d. While these subgroups are thought to play a role in non-methane hydrocarbon degradation, there is little genomic information available to confirm their metabolic function. As such, these novel single-cell amplified genomes provide valuable insight into the metabolic potential of this prevalent subseafloor clade.

AB - The SEEP-SRB1 is a diverse clade of sulfate reducing bacteria affiliated with the deltaproteobacterial family Desulfobacteraceae. Here we report the draft genome sequences of two single cells belonging to the SEEP-SRB1 clade, isolated from marine sediment in Aarhus Bay, Denmark, by fluorescence activated cell sorting (FACS). The draft genomes measure 2.12 Mb and 0.46 Mb, with GC contents of 45% and 42%. Phylogenetic placement of the 16S rRNA genes places the SAGs within the subgroups SEEP-SRB1e and SEEP-SRB1d. While these subgroups are thought to play a role in non-methane hydrocarbon degradation, there is little genomic information available to confirm their metabolic function. As such, these novel single-cell amplified genomes provide valuable insight into the metabolic potential of this prevalent subseafloor clade.

KW - Hydrocarbon degradation

KW - SAG

KW - SEEP-SRB

KW - Single-cell genomics

KW - Sulfate-reducing bacteria

KW - ARCHAEA

KW - ANAEROBIC OXIDATION

KW - PHYLOGENETIC ANALYSIS

KW - REDUCTASE

KW - DIVERSITY

KW - SULFATE-REDUCING BACTERIA

KW - TOOL

UR - http://www.scopus.com/inward/record.url?scp=85060434618&partnerID=8YFLogxK

U2 - 10.1016/j.margen.2019.01.004

DO - 10.1016/j.margen.2019.01.004

M3 - Journal article

AN - SCOPUS:85060434618

VL - 46

SP - 66

EP - 69

JO - Marine Genomics

JF - Marine Genomics

SN - 1874-7787

ER -