Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment

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  • Caitlin Petro, School of Biological Sciences, Georgia Institute of Technology
  • ,
  • Lara M. Jochum, Max von Pettenkofer Institute for Hygiene and Clinical Microbiology, Ludwig-Maximilians-University München
  • ,
  • Lars Schreiber, National Research Council Canada
  • ,
  • Ian P.G. Marshall
  • Andreas Schramm
  • Kasper U. Kjeldsen

The SEEP-SRB1 is a diverse clade of sulfate reducing bacteria affiliated with the deltaproteobacterial family Desulfobacteraceae. Here we report the draft genome sequences of two single cells belonging to the SEEP-SRB1 clade, isolated from marine sediment in Aarhus Bay, Denmark, by fluorescence activated cell sorting (FACS). The draft genomes measure 2.12 Mb and 0.46 Mb, with GC contents of 45% and 42%. Phylogenetic placement of the 16S rRNA genes places the SAGs within the subgroups SEEP-SRB1e and SEEP-SRB1d. While these subgroups are thought to play a role in non-methane hydrocarbon degradation, there is little genomic information available to confirm their metabolic function. As such, these novel single-cell amplified genomes provide valuable insight into the metabolic potential of this prevalent subseafloor clade.

OriginalsprogEngelsk
TidsskriftMarine Genomics
ISSN1874-7787
DOI
StatusAccepteret/In press - 1 jan. 2019

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