Single cell enzyme diagnosis on the chip

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisKonferenceartikelForskningpeer review

Standard

Single cell enzyme diagnosis on the chip. / Jensen, Sissel Juul; Harmsen, Charlotte; Nielsen, Mette Juul; Stougaard, Magnus; Leong, Kam W; Knudsen, Birgitta R; Ho, Yi-Ping.

I: International Conference on Micro Electro Mechanical Systems, 20.01.2013, s. 911 - 914.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisKonferenceartikelForskningpeer review

Harvard

Jensen, SJ, Harmsen, C, Nielsen, MJ, Stougaard, M, Leong, KW, Knudsen, BR & Ho, Y-P 2013, 'Single cell enzyme diagnosis on the chip', International Conference on Micro Electro Mechanical Systems, s. 911 - 914. https://doi.org/10.1109/MEMSYS.2013.6474392

APA

Jensen, S. J., Harmsen, C., Nielsen, M. J., Stougaard, M., Leong, K. W., Knudsen, B. R., & Ho, Y-P. (2013). Single cell enzyme diagnosis on the chip. International Conference on Micro Electro Mechanical Systems, 911 - 914. https://doi.org/10.1109/MEMSYS.2013.6474392

CBE

Jensen SJ, Harmsen C, Nielsen MJ, Stougaard M, Leong KW, Knudsen BR, Ho Y-P. 2013. Single cell enzyme diagnosis on the chip. International Conference on Micro Electro Mechanical Systems. 911 - 914. https://doi.org/10.1109/MEMSYS.2013.6474392

MLA

Jensen, Sissel Juul o.a.. "Single cell enzyme diagnosis on the chip". International Conference on Micro Electro Mechanical Systems. 2013, 911 - 914. https://doi.org/10.1109/MEMSYS.2013.6474392

Vancouver

Jensen SJ, Harmsen C, Nielsen MJ, Stougaard M, Leong KW, Knudsen BR o.a. Single cell enzyme diagnosis on the chip. International Conference on Micro Electro Mechanical Systems. 2013 jan 20;911 - 914. https://doi.org/10.1109/MEMSYS.2013.6474392

Author

Jensen, Sissel Juul ; Harmsen, Charlotte ; Nielsen, Mette Juul ; Stougaard, Magnus ; Leong, Kam W ; Knudsen, Birgitta R ; Ho, Yi-Ping. / Single cell enzyme diagnosis on the chip. I: International Conference on Micro Electro Mechanical Systems. 2013 ; s. 911 - 914.

Bibtex

@inproceedings{aac8a1ada9924e7db754983fcb267dcf,
title = "Single cell enzyme diagnosis on the chip",
abstract = "Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms",
author = "Jensen, {Sissel Juul} and Charlotte Harmsen and Nielsen, {Mette Juul} and Magnus Stougaard and Leong, {Kam W} and Knudsen, {Birgitta R} and Yi-Ping Ho",
year = "2013",
month = jan,
day = "20",
doi = "10.1109/MEMSYS.2013.6474392",
language = "English",
pages = "911 -- 914",
journal = "International Conference on Micro Electro Mechanical Systems",
issn = "1084-6999",
publisher = "I E E E ",
note = "Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on ; Conference date: 20-01-2013 Through 24-01-2013",

}

RIS

TY - GEN

T1 - Single cell enzyme diagnosis on the chip

AU - Jensen, Sissel Juul

AU - Harmsen, Charlotte

AU - Nielsen, Mette Juul

AU - Stougaard, Magnus

AU - Leong, Kam W

AU - Knudsen, Birgitta R

AU - Ho, Yi-Ping

PY - 2013/1/20

Y1 - 2013/1/20

N2 - Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms

AB - Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms

UR - http://www.scopus.com/inward/record.url?scp=84875473876&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2013.6474392

DO - 10.1109/MEMSYS.2013.6474392

M3 - Conference article

AN - SCOPUS:84875473876

SP - 911

EP - 914

JO - International Conference on Micro Electro Mechanical Systems

JF - International Conference on Micro Electro Mechanical Systems

SN - 1084-6999

T2 - Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on

Y2 - 20 January 2013 through 24 January 2013

ER -