Single cell enzyme diagnosis on the chip

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  • Sissel Juul Jensen
  • ,
  • Charlotte Harmsen
  • ,
  • Mette Juul Nielsen, Aarhus Universitet, Danmark
  • Magnus Stougaard
  • Kam W Leong, Duke University, USA
  • Birgitta R Knudsen
  • Yi-Ping Ho, Danmark
Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms
OriginalsprogEngelsk
TidsskriftInternational Conference on Micro Electro Mechanical Systems
Sider (fra-til)911 - 914
Antal sider4
ISSN1084-6999
DOI
StatusUdgivet - 20 jan. 2013
BegivenhedMicro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on - Taipei, Taiwan
Varighed: 20 jan. 201324 jan. 2013

Konference

KonferenceMicro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on
LandTaiwan
ByTaipei
Periode20/01/201324/01/2013

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