Simple and reliable procedure for PCR amplification of genomic DNA from yeast cells using short sequencing primers.

J Haaning; C Oxvig; Michael Toft Overgaard; L Sottrup-Jensen

Simple and reliable procedure for PCR amplification of genomic DNA from yeast cells using short sequencing primers.

J Haaning
, C Oxvig
, Michael Toft Overgaard
, L Sottrup-Jensen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

12 Citationer (Scopus)

Abstract

Yeast is widely used in molecular biology. Heterologous expression of recombinant proteins in yeast involves screening of a large number of recombinants. We present an easy and reliable procedure for amplifying genomic DNA from freshly grown cells of the methylotrophic yeast Pichia pastoris by means of PCR without any prior DNA purification steps. This method involves a simple boiling step of whole yeast cells in the presence of detergent, and subsequent amplification of genomic DNA using short sequencing primers in a polymerase chain reaction assay with a decreasing annealing temperature.
Udgivelsesdato: 1997-Jun

Originalsprog	Engelsk
Tidsskrift	IUBMB Life
Vol/bind	42
Nummer	1
Sider (fra-til)	169-72
Antal sider	3
ISSN	1521-6543
Status	Udgivet - 1997

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title = "Simple and reliable procedure for PCR amplification of genomic DNA from yeast cells using short sequencing primers.",

abstract = "Yeast is widely used in molecular biology. Heterologous expression of recombinant proteins in yeast involves screening of a large number of recombinants. We present an easy and reliable procedure for amplifying genomic DNA from freshly grown cells of the methylotrophic yeast Pichia pastoris by means of PCR without any prior DNA purification steps. This method involves a simple boiling step of whole yeast cells in the presence of detergent, and subsequent amplification of genomic DNA using short sequencing primers in a polymerase chain reaction assay with a decreasing annealing temperature. Udgivelsesdato: 1997-Jun",

keywords = "Base Sequence, DNA Primers, DNA, Fungal, Detergents, Heat, Pichia, Polymerase Chain Reaction, Templates, Genetic",

author = "J Haaning and C Oxvig and Overgaard, \{Michael Toft\} and L Sottrup-Jensen",

year = "1997",

language = "English",

volume = "42",

pages = "169--72",

journal = "IUBMB Life",

issn = "1521-6543",

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T1 - Simple and reliable procedure for PCR amplification of genomic DNA from yeast cells using short sequencing primers.

AU - Haaning, J

AU - Oxvig, C

AU - Overgaard, Michael Toft

AU - Sottrup-Jensen, L

PY - 1997

Y1 - 1997

N2 - Yeast is widely used in molecular biology. Heterologous expression of recombinant proteins in yeast involves screening of a large number of recombinants. We present an easy and reliable procedure for amplifying genomic DNA from freshly grown cells of the methylotrophic yeast Pichia pastoris by means of PCR without any prior DNA purification steps. This method involves a simple boiling step of whole yeast cells in the presence of detergent, and subsequent amplification of genomic DNA using short sequencing primers in a polymerase chain reaction assay with a decreasing annealing temperature. Udgivelsesdato: 1997-Jun

AB - Yeast is widely used in molecular biology. Heterologous expression of recombinant proteins in yeast involves screening of a large number of recombinants. We present an easy and reliable procedure for amplifying genomic DNA from freshly grown cells of the methylotrophic yeast Pichia pastoris by means of PCR without any prior DNA purification steps. This method involves a simple boiling step of whole yeast cells in the presence of detergent, and subsequent amplification of genomic DNA using short sequencing primers in a polymerase chain reaction assay with a decreasing annealing temperature. Udgivelsesdato: 1997-Jun

KW - Base Sequence

KW - DNA Primers

KW - DNA, Fungal

KW - Detergents

KW - Heat

KW - Pichia

KW - Polymerase Chain Reaction

KW - Templates, Genetic

M3 - Journal article

C2 - 9192097

SN - 1521-6543

VL - 42

SP - 169

EP - 172

JO - IUBMB Life

JF - IUBMB Life

IS - 1

ER -