Siberian Miscanthus sacchariflorus accessions surpass the exceptional chilling tolerance of the most widely cultivated clone of Miscanthus x giganteus

Charles P. Pignon; Idan Spitz; Erik J. Sacks; Uffe Jørgensen; Kirsten Kørup; Stephen P. Long

doi:10.1111/gcbb.12599

Siberian Miscanthus sacchariflorus accessions surpass the exceptional chilling tolerance of the most widely cultivated clone of Miscanthus x giganteus

Charles P. Pignon, Idan Spitz, Erik J. Sacks, Uffe Jørgensen, Kirsten Kørup, Stephen P. Long^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

Chilling temperatures (0–15°C) inhibit photosynthesis in most C₄ grasses, yet photosynthesis is chilling tolerant in the ‘Illinois’ clone of the C₄ grass Miscanthus x giganteus, a candidate cellulosic bioenergy crop. M. x giganteus is a hybrid between Miscanthus sacchariflorus and Miscanthus sinensis; therefore chilling-tolerant parent lines might produce hybrids superior to the current clone. Recently a collection of M. sacchariflorus from Siberia, the apparent low temperature limit of natural distribution, became available, which may be a source for chilling tolerance. The collection was screened for chilling tolerance of photosynthesis by measuring dark-adapted maximum quantum yield of PSII photochemistry (F_v/F_m) on plants in the field in cool weather. Superior accessions were selected for further phenotyping: plants were grown at 25°C, transferred to 10°C (chilling) for 15 days, and returned to 25°C for 7 days (recovery). Two experiments assessed: (a) light-saturated net photosynthetic rate (A_sat) and operating quantum yield of PSII photochemistry (Φ_PSII), (b) response of net leaf CO₂ uptake (A) to intercellular [CO₂] (c_i). Three accessions showed superior chilling tolerance: RU2012-069 and RU2012-114 achieved A_sat up to double that of M. x giganteus prior to and during chilling, due to increased c_i - saturated photosynthesis (V_max). RU2012-069 and RU2012-114 also maintained greater levels of Φ_PSII during chilling, indicating reduced photodamage. Additionally, accession RU2012-112 maintained a stable A_sat throughout the 15-day chilling period, while A_sat continuously declined in other accessions; this suggests RU2012-112 could outperform others in lengthy chilling periods. Plants were returned to 25°C after the chilling period; M. x giganteus showed the weakest recovery after 1 day, but a strong recovery after 1 week. This study has therefore identified important genetic resources for the synthesis of improved lines of M. x giganteus, which could facilitate the displacement of fossil fuels by cellulosic bioenergy.

Originalsprog	Engelsk
Tidsskrift	GCB Bioenergy
Vol/bind	11
Nummer	7
Sider (fra-til)	883-894
Antal sider	12
ISSN	1757-1693
DOI	https://doi.org/10.1111/gcbb.12599
Status	Udgivet - jul. 2019

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@article{ee1668f5223345be858084eae7ed5871,

title = "Siberian Miscanthus sacchariflorus accessions surpass the exceptional chilling tolerance of the most widely cultivated clone of Miscanthus x giganteus",

abstract = "Chilling temperatures (0–15°C) inhibit photosynthesis in most C4 grasses, yet photosynthesis is chilling tolerant in the {\textquoteleft}Illinois{\textquoteright} clone of the C4 grass Miscanthus x giganteus, a candidate cellulosic bioenergy crop. M. x giganteus is a hybrid between Miscanthus sacchariflorus and Miscanthus sinensis; therefore chilling-tolerant parent lines might produce hybrids superior to the current clone. Recently a collection of M. sacchariflorus from Siberia, the apparent low temperature limit of natural distribution, became available, which may be a source for chilling tolerance. The collection was screened for chilling tolerance of photosynthesis by measuring dark-adapted maximum quantum yield of PSII photochemistry (Fv/Fm) on plants in the field in cool weather. Superior accessions were selected for further phenotyping: plants were grown at 25°C, transferred to 10°C (chilling) for 15 days, and returned to 25°C for 7 days (recovery). Two experiments assessed: (a) light-saturated net photosynthetic rate (Asat) and operating quantum yield of PSII photochemistry (ΦPSII), (b) response of net leaf CO2 uptake (A) to intercellular [CO2] (ci). Three accessions showed superior chilling tolerance: RU2012-069 and RU2012-114 achieved Asat up to double that of M. x giganteus prior to and during chilling, due to increased ci - saturated photosynthesis (Vmax). RU2012-069 and RU2012-114 also maintained greater levels of ΦPSII during chilling, indicating reduced photodamage. Additionally, accession RU2012-112 maintained a stable Asat throughout the 15-day chilling period, while Asat continuously declined in other accessions; this suggests RU2012-112 could outperform others in lengthy chilling periods. Plants were returned to 25°C after the chilling period; M. x giganteus showed the weakest recovery after 1 day, but a strong recovery after 1 week. This study has therefore identified important genetic resources for the synthesis of improved lines of M. x giganteus, which could facilitate the displacement of fossil fuels by cellulosic bioenergy.",

keywords = "bioenergy, biomass, chilling tolerance, cold tolerance, Miscanthus sacchariflorus, Miscanthus x giganteus, photosynthesis, photosystem II, Siberia",

author = "Pignon, {Charles P.} and Idan Spitz and Sacks, {Erik J.} and Uffe J{\o}rgensen and Kirsten K{\o}rup and Long, {Stephen P.}",

year = "2019",

month = jul,

doi = "10.1111/gcbb.12599",

language = "English",

volume = "11",

pages = "883--894",

journal = "GCB Bioenergy",

issn = "1757-1693",

publisher = "John Wiley Sons Ltd",

number = "7",

}

Siberian Miscanthus sacchariflorus accessions surpass the exceptional chilling tolerance of the most widely cultivated clone of Miscanthus x giganteus. / Pignon, Charles P.; Spitz, Idan; Sacks, Erik J. et al.
I: GCB Bioenergy, Bind 11, Nr. 7, 07.2019, s. 883-894.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Siberian Miscanthus sacchariflorus accessions surpass the exceptional chilling tolerance of the most widely cultivated clone of Miscanthus x giganteus

AU - Pignon, Charles P.

AU - Spitz, Idan

AU - Sacks, Erik J.

AU - Jørgensen, Uffe

AU - Kørup, Kirsten

AU - Long, Stephen P.

PY - 2019/7

Y1 - 2019/7

N2 - Chilling temperatures (0–15°C) inhibit photosynthesis in most C4 grasses, yet photosynthesis is chilling tolerant in the ‘Illinois’ clone of the C4 grass Miscanthus x giganteus, a candidate cellulosic bioenergy crop. M. x giganteus is a hybrid between Miscanthus sacchariflorus and Miscanthus sinensis; therefore chilling-tolerant parent lines might produce hybrids superior to the current clone. Recently a collection of M. sacchariflorus from Siberia, the apparent low temperature limit of natural distribution, became available, which may be a source for chilling tolerance. The collection was screened for chilling tolerance of photosynthesis by measuring dark-adapted maximum quantum yield of PSII photochemistry (Fv/Fm) on plants in the field in cool weather. Superior accessions were selected for further phenotyping: plants were grown at 25°C, transferred to 10°C (chilling) for 15 days, and returned to 25°C for 7 days (recovery). Two experiments assessed: (a) light-saturated net photosynthetic rate (Asat) and operating quantum yield of PSII photochemistry (ΦPSII), (b) response of net leaf CO2 uptake (A) to intercellular [CO2] (ci). Three accessions showed superior chilling tolerance: RU2012-069 and RU2012-114 achieved Asat up to double that of M. x giganteus prior to and during chilling, due to increased ci - saturated photosynthesis (Vmax). RU2012-069 and RU2012-114 also maintained greater levels of ΦPSII during chilling, indicating reduced photodamage. Additionally, accession RU2012-112 maintained a stable Asat throughout the 15-day chilling period, while Asat continuously declined in other accessions; this suggests RU2012-112 could outperform others in lengthy chilling periods. Plants were returned to 25°C after the chilling period; M. x giganteus showed the weakest recovery after 1 day, but a strong recovery after 1 week. This study has therefore identified important genetic resources for the synthesis of improved lines of M. x giganteus, which could facilitate the displacement of fossil fuels by cellulosic bioenergy.

AB - Chilling temperatures (0–15°C) inhibit photosynthesis in most C4 grasses, yet photosynthesis is chilling tolerant in the ‘Illinois’ clone of the C4 grass Miscanthus x giganteus, a candidate cellulosic bioenergy crop. M. x giganteus is a hybrid between Miscanthus sacchariflorus and Miscanthus sinensis; therefore chilling-tolerant parent lines might produce hybrids superior to the current clone. Recently a collection of M. sacchariflorus from Siberia, the apparent low temperature limit of natural distribution, became available, which may be a source for chilling tolerance. The collection was screened for chilling tolerance of photosynthesis by measuring dark-adapted maximum quantum yield of PSII photochemistry (Fv/Fm) on plants in the field in cool weather. Superior accessions were selected for further phenotyping: plants were grown at 25°C, transferred to 10°C (chilling) for 15 days, and returned to 25°C for 7 days (recovery). Two experiments assessed: (a) light-saturated net photosynthetic rate (Asat) and operating quantum yield of PSII photochemistry (ΦPSII), (b) response of net leaf CO2 uptake (A) to intercellular [CO2] (ci). Three accessions showed superior chilling tolerance: RU2012-069 and RU2012-114 achieved Asat up to double that of M. x giganteus prior to and during chilling, due to increased ci - saturated photosynthesis (Vmax). RU2012-069 and RU2012-114 also maintained greater levels of ΦPSII during chilling, indicating reduced photodamage. Additionally, accession RU2012-112 maintained a stable Asat throughout the 15-day chilling period, while Asat continuously declined in other accessions; this suggests RU2012-112 could outperform others in lengthy chilling periods. Plants were returned to 25°C after the chilling period; M. x giganteus showed the weakest recovery after 1 day, but a strong recovery after 1 week. This study has therefore identified important genetic resources for the synthesis of improved lines of M. x giganteus, which could facilitate the displacement of fossil fuels by cellulosic bioenergy.

KW - bioenergy

KW - biomass

KW - chilling tolerance

KW - cold tolerance

KW - Miscanthus sacchariflorus

KW - Miscanthus x giganteus

KW - photosynthesis

KW - photosystem II

KW - Siberia

UR - http://www.scopus.com/inward/record.url?scp=85067835898&partnerID=8YFLogxK

U2 - 10.1111/gcbb.12599

DO - 10.1111/gcbb.12599

M3 - Journal article

AN - SCOPUS:85067835898

SN - 1757-1693

VL - 11

SP - 883

EP - 894

JO - GCB Bioenergy

JF - GCB Bioenergy

IS - 7

ER -

Siberian Miscanthus sacchariflorus accessions surpass the exceptional chilling tolerance of the most widely cultivated clone of Miscanthus x giganteus

Abstract

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