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Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells

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Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells. / Furlanetto, Ana L.D.M.; Cadena, Silvia M.S.C.; Martinez, Glaucia R.; Ferrando, Beatriz; Stevnsner, Tinna; Møller, Ian M.

I: Physiologia Plantarum, Bind 166, Nr. 2, 06.2019, s. 513-524.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Furlanetto, Ana L.D.M. ; Cadena, Silvia M.S.C. ; Martinez, Glaucia R. ; Ferrando, Beatriz ; Stevnsner, Tinna ; Møller, Ian M. / Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells. I: Physiologia Plantarum. 2019 ; Bind 166, Nr. 2. s. 513-524.

Bibtex

@article{081e010010ac40ab9a96aaa2ec3416b1,
title = "Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells",
abstract = "We evaluated the effect of global warming on Araucaria angustifolia (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40{\%} after incubation at 30 and 37°C for 24 and 6 h, respectively, while 2 h at 40 and 42°C killed 95{\%} of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2 h at 42°C. The in vitro activity of enzymes of the base excision repair (BER) pathway was determined. Apurinic/apyrimidine endonuclease, measured in extracts from cells incubated for 2 h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30-min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited at 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and 37°C, and that they are all very sensitive to 40 or 42°C. Thus, A. angustifolia will likely be vulnerable to the short-term high temperature events associated with global warming.",
keywords = "BASE EXCISION-REPAIR, CHLOROPLASTS, KUNTZE, METABOLISM, OXIDATIVE STRESS, PLANT-MITOCHONDRIA, REACTIVE OXYGEN, RESPONSES, SOMATIC EMBRYOGENESIS, TOLERANCE, DNA-(Apurinic or Apyrimidinic Site) Lyase/genetics, Temperature, Tracheophyta/enzymology, DNA Repair/genetics, Cell Nucleus/genetics, DNA Repair Enzymes/genetics",
author = "Furlanetto, {Ana L.D.M.} and Cadena, {Silvia M.S.C.} and Martinez, {Glaucia R.} and Beatriz Ferrando and Tinna Stevnsner and M{\o}ller, {Ian M.}",
year = "2019",
month = "6",
doi = "10.1111/ppl.12793",
language = "English",
volume = "166",
pages = "513--524",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell Publishing, Inc",
number = "2",

}

RIS

TY - JOUR

T1 - Short-term high temperature treatment reduces viability and inhibits respiration and DNA repair enzymes in Araucaria angustifolia cells

AU - Furlanetto, Ana L.D.M.

AU - Cadena, Silvia M.S.C.

AU - Martinez, Glaucia R.

AU - Ferrando, Beatriz

AU - Stevnsner, Tinna

AU - Møller, Ian M.

PY - 2019/6

Y1 - 2019/6

N2 - We evaluated the effect of global warming on Araucaria angustifolia (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40% after incubation at 30 and 37°C for 24 and 6 h, respectively, while 2 h at 40 and 42°C killed 95% of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2 h at 42°C. The in vitro activity of enzymes of the base excision repair (BER) pathway was determined. Apurinic/apyrimidine endonuclease, measured in extracts from cells incubated for 2 h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30-min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited at 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and 37°C, and that they are all very sensitive to 40 or 42°C. Thus, A. angustifolia will likely be vulnerable to the short-term high temperature events associated with global warming.

AB - We evaluated the effect of global warming on Araucaria angustifolia (Bert.) O. Kuntze, a critically endangered native tree of Southern Brazil, by studying the effects of short-term high temperature treatment on cell viability, respiration and DNA repair of embryogenic cells. Compared with control cells grown at 25°C, cell viability was reduced by 40% after incubation at 30 and 37°C for 24 and 6 h, respectively, while 2 h at 40 and 42°C killed 95% of the cells. Cell respiration was unaffected at 30-37°C, but dramatically reduced after 2 h at 42°C. The in vitro activity of enzymes of the base excision repair (BER) pathway was determined. Apurinic/apyrimidine endonuclease, measured in extracts from cells incubated for 2 h at 42°C, was completely inactivated while lower temperatures had no effect. The activities of three enzymes of the mitochondrial BER pathway were measured after 30-min preincubation of isolated mitochondria at 25-40°C and one of them, uracil glycosylase, was completely inhibited at 40°C. We conclude that cell viability, respiration and DNA repair have different temperature sensitivities between 25 and 37°C, and that they are all very sensitive to 40 or 42°C. Thus, A. angustifolia will likely be vulnerable to the short-term high temperature events associated with global warming.

KW - BASE EXCISION-REPAIR

KW - CHLOROPLASTS

KW - KUNTZE

KW - METABOLISM

KW - OXIDATIVE STRESS

KW - PLANT-MITOCHONDRIA

KW - REACTIVE OXYGEN

KW - RESPONSES

KW - SOMATIC EMBRYOGENESIS

KW - TOLERANCE

KW - DNA-(Apurinic or Apyrimidinic Site) Lyase/genetics

KW - Temperature

KW - Tracheophyta/enzymology

KW - DNA Repair/genetics

KW - Cell Nucleus/genetics

KW - DNA Repair Enzymes/genetics

UR - http://www.scopus.com/inward/record.url?scp=85053418443&partnerID=8YFLogxK

U2 - 10.1111/ppl.12793

DO - 10.1111/ppl.12793

M3 - Journal article

C2 - 29952010

AN - SCOPUS:85053418443

VL - 166

SP - 513

EP - 524

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 2

ER -