Sequence variation in the alpha-toxin encoding plc gene of Clostridium perfringens strains isolated from diseased and healthy chickens

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Sequence variation in the alpha-toxin encoding plc gene of Clostridium perfringens strains isolated from diseased and healthy chickens. / Abildgaard, Lone; Engberg, Ricarda M; Pedersen, Karl; Schramm, Andreas; Hojberg, Ole.

I: Veterinary Microbiology, Bind 136, Nr. 3-4, 2009, s. 293-299.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{2cc1b7e0f82e11dd8b7a000ea68e967b,
title = "Sequence variation in the alpha-toxin encoding plc gene of Clostridium perfringens strains isolated from diseased and healthy chickens",
abstract = "The aim of the present study was to analyse the genetic diversity of the alpha-toxin encoding plc gene and the variation in alpha-toxin production of Clostridium perfringens type A strains isolated from presumably healthy chickens and chickens suffering from either necrotic enteritis (NE) or cholangio-hepatitis. The alpha-toxin encoding plc genes from 60 different pulsed-field gel electrophoresis (PFGE) types (strains) of C. perfringens were sequenced and translated in silico to amino acid sequences and the alpha-toxin production was investigated in batch cultures of 45 of the strains using an enzyme-linked immunosorbent assay (ELISA) approach. Overall, the truncated amino acid sequences showed close similarity (>98{\%} at the amino acid level) to previously reported sequences from chicken-derived C. perfringens isolates. Variations were however observed in 23 out of 379 aa positions leading to the definition of 26 different alpha-toxin sequence types among the 60 strains. Moreover, a type II intron of 834 non-coding nucleotides was identified in the plc gene of three of the investigated strains. The in vitro alpha-toxin production investigated in 45 of the strains, including the three harbouring the intron, revealed no correlation between PFGE type, alpha-toxin sequence type, health status of the host chickens and level of alpha-toxin production. It is therefore concluded that neither plc gene type nor alpha-toxin production level seems to correlate to origin (healthy or diseased chicken) of the C. perfringens strains.",
keywords = "α-Toxin, C. perfringens, plc gene",
author = "Lone Abildgaard and Engberg, {Ricarda M} and Karl Pedersen and Andreas Schramm and Ole Hojberg",
year = "2009",
doi = "10.1016/j.vetmic.2008.11.001",
language = "English",
volume = "136",
pages = "293--299",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier BV",
number = "3-4",

}

RIS

TY - JOUR

T1 - Sequence variation in the alpha-toxin encoding plc gene of Clostridium perfringens strains isolated from diseased and healthy chickens

AU - Abildgaard, Lone

AU - Engberg, Ricarda M

AU - Pedersen, Karl

AU - Schramm, Andreas

AU - Hojberg, Ole

PY - 2009

Y1 - 2009

N2 - The aim of the present study was to analyse the genetic diversity of the alpha-toxin encoding plc gene and the variation in alpha-toxin production of Clostridium perfringens type A strains isolated from presumably healthy chickens and chickens suffering from either necrotic enteritis (NE) or cholangio-hepatitis. The alpha-toxin encoding plc genes from 60 different pulsed-field gel electrophoresis (PFGE) types (strains) of C. perfringens were sequenced and translated in silico to amino acid sequences and the alpha-toxin production was investigated in batch cultures of 45 of the strains using an enzyme-linked immunosorbent assay (ELISA) approach. Overall, the truncated amino acid sequences showed close similarity (>98% at the amino acid level) to previously reported sequences from chicken-derived C. perfringens isolates. Variations were however observed in 23 out of 379 aa positions leading to the definition of 26 different alpha-toxin sequence types among the 60 strains. Moreover, a type II intron of 834 non-coding nucleotides was identified in the plc gene of three of the investigated strains. The in vitro alpha-toxin production investigated in 45 of the strains, including the three harbouring the intron, revealed no correlation between PFGE type, alpha-toxin sequence type, health status of the host chickens and level of alpha-toxin production. It is therefore concluded that neither plc gene type nor alpha-toxin production level seems to correlate to origin (healthy or diseased chicken) of the C. perfringens strains.

AB - The aim of the present study was to analyse the genetic diversity of the alpha-toxin encoding plc gene and the variation in alpha-toxin production of Clostridium perfringens type A strains isolated from presumably healthy chickens and chickens suffering from either necrotic enteritis (NE) or cholangio-hepatitis. The alpha-toxin encoding plc genes from 60 different pulsed-field gel electrophoresis (PFGE) types (strains) of C. perfringens were sequenced and translated in silico to amino acid sequences and the alpha-toxin production was investigated in batch cultures of 45 of the strains using an enzyme-linked immunosorbent assay (ELISA) approach. Overall, the truncated amino acid sequences showed close similarity (>98% at the amino acid level) to previously reported sequences from chicken-derived C. perfringens isolates. Variations were however observed in 23 out of 379 aa positions leading to the definition of 26 different alpha-toxin sequence types among the 60 strains. Moreover, a type II intron of 834 non-coding nucleotides was identified in the plc gene of three of the investigated strains. The in vitro alpha-toxin production investigated in 45 of the strains, including the three harbouring the intron, revealed no correlation between PFGE type, alpha-toxin sequence type, health status of the host chickens and level of alpha-toxin production. It is therefore concluded that neither plc gene type nor alpha-toxin production level seems to correlate to origin (healthy or diseased chicken) of the C. perfringens strains.

KW - α-Toxin

KW - C. perfringens

KW - plc gene

U2 - 10.1016/j.vetmic.2008.11.001

DO - 10.1016/j.vetmic.2008.11.001

M3 - Journal article

C2 - 19070974

VL - 136

SP - 293

EP - 299

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -