Self-assembly of a nanoscale DNA box with a controllable lid

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Self-assembly of a nanoscale DNA box with a controllable lid. / Andersen, Ebbe S; Dong, Mingdong; Nielsen, Morten Muhlig; Jahn, Kasper; Subramani, Ramesh; Mamdouh, Wael; Golas, Monika M; Sander, Bjoern; Stark, Holger; Oliveira, Cristiano L P; Pedersen, Jan Skov; Birkedal, Victoria; Besenbacher, Flemming; Gothelf, Kurt V; Kjems, Jørgen.

I: Nature, Bind 459, Nr. 7243, 2009, s. 73-6.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Andersen, Ebbe S ; Dong, Mingdong ; Nielsen, Morten Muhlig ; Jahn, Kasper ; Subramani, Ramesh ; Mamdouh, Wael ; Golas, Monika M ; Sander, Bjoern ; Stark, Holger ; Oliveira, Cristiano L P ; Pedersen, Jan Skov ; Birkedal, Victoria ; Besenbacher, Flemming ; Gothelf, Kurt V ; Kjems, Jørgen. / Self-assembly of a nanoscale DNA box with a controllable lid. I: Nature. 2009 ; Bind 459, Nr. 7243. s. 73-6.

Bibtex

@article{54089ca0726811df8c1a000ea68e967b,
title = "Self-assembly of a nanoscale DNA box with a controllable lid",
abstract = "The unique structural motifs and self-recognition properties of DNA can be exploited to generate self-assembling DNA nanostructures of specific shapes using a 'bottom-up' approach. Several assembly strategies have been developed for building complex three-dimensional (3D) DNA nanostructures. Recently, the DNA 'origami' method was used to build two-dimensional addressable DNA structures of arbitrary shape that can be used as platforms to arrange nanomaterials with high precision and specificity. A long-term goal of this field has been to construct fully addressable 3D DNA nanostructures. Here we extend the DNA origami method into three dimensions by creating an addressable DNA box 42 x 36 x 36 nm(3) in size that can be opened in the presence of externally supplied DNA 'keys'. We thoroughly characterize the structure of this DNA box using cryogenic transmission electron microscopy, small-angle X-ray scattering and atomic force microscopy, and use fluorescence resonance energy transfer to optically monitor the opening of the lid. Controlled access to the interior compartment of this DNA nanocontainer could yield several interesting applications, for example as a logic sensor for multiple-sequence signals or for the controlled release of nanocargos.",
keywords = "Cryoelectron Microscopy, DNA, Imaging, Three-Dimensional, Microscopy, Atomic Force, Nanostructures, Nucleic Acid Conformation",
author = "Andersen, {Ebbe S} and Mingdong Dong and Nielsen, {Morten Muhlig} and Kasper Jahn and Ramesh Subramani and Wael Mamdouh and Golas, {Monika M} and Bjoern Sander and Holger Stark and Oliveira, {Cristiano L P} and Pedersen, {Jan Skov} and Victoria Birkedal and Flemming Besenbacher and Gothelf, {Kurt V} and J{\o}rgen Kjems",
year = "2009",
doi = "10.1038/nature07971",
language = "English",
volume = "459",
pages = "73--6",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7243",

}

RIS

TY - JOUR

T1 - Self-assembly of a nanoscale DNA box with a controllable lid

AU - Andersen, Ebbe S

AU - Dong, Mingdong

AU - Nielsen, Morten Muhlig

AU - Jahn, Kasper

AU - Subramani, Ramesh

AU - Mamdouh, Wael

AU - Golas, Monika M

AU - Sander, Bjoern

AU - Stark, Holger

AU - Oliveira, Cristiano L P

AU - Pedersen, Jan Skov

AU - Birkedal, Victoria

AU - Besenbacher, Flemming

AU - Gothelf, Kurt V

AU - Kjems, Jørgen

PY - 2009

Y1 - 2009

N2 - The unique structural motifs and self-recognition properties of DNA can be exploited to generate self-assembling DNA nanostructures of specific shapes using a 'bottom-up' approach. Several assembly strategies have been developed for building complex three-dimensional (3D) DNA nanostructures. Recently, the DNA 'origami' method was used to build two-dimensional addressable DNA structures of arbitrary shape that can be used as platforms to arrange nanomaterials with high precision and specificity. A long-term goal of this field has been to construct fully addressable 3D DNA nanostructures. Here we extend the DNA origami method into three dimensions by creating an addressable DNA box 42 x 36 x 36 nm(3) in size that can be opened in the presence of externally supplied DNA 'keys'. We thoroughly characterize the structure of this DNA box using cryogenic transmission electron microscopy, small-angle X-ray scattering and atomic force microscopy, and use fluorescence resonance energy transfer to optically monitor the opening of the lid. Controlled access to the interior compartment of this DNA nanocontainer could yield several interesting applications, for example as a logic sensor for multiple-sequence signals or for the controlled release of nanocargos.

AB - The unique structural motifs and self-recognition properties of DNA can be exploited to generate self-assembling DNA nanostructures of specific shapes using a 'bottom-up' approach. Several assembly strategies have been developed for building complex three-dimensional (3D) DNA nanostructures. Recently, the DNA 'origami' method was used to build two-dimensional addressable DNA structures of arbitrary shape that can be used as platforms to arrange nanomaterials with high precision and specificity. A long-term goal of this field has been to construct fully addressable 3D DNA nanostructures. Here we extend the DNA origami method into three dimensions by creating an addressable DNA box 42 x 36 x 36 nm(3) in size that can be opened in the presence of externally supplied DNA 'keys'. We thoroughly characterize the structure of this DNA box using cryogenic transmission electron microscopy, small-angle X-ray scattering and atomic force microscopy, and use fluorescence resonance energy transfer to optically monitor the opening of the lid. Controlled access to the interior compartment of this DNA nanocontainer could yield several interesting applications, for example as a logic sensor for multiple-sequence signals or for the controlled release of nanocargos.

KW - Cryoelectron Microscopy

KW - DNA

KW - Imaging, Three-Dimensional

KW - Microscopy, Atomic Force

KW - Nanostructures

KW - Nucleic Acid Conformation

U2 - 10.1038/nature07971

DO - 10.1038/nature07971

M3 - Journal article

C2 - 19424153

VL - 459

SP - 73

EP - 76

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7243

ER -