Selection of antibodies against a single rare cell present in a heterogeneous population using phage display

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Selection of antibodies against a single rare cell present in a heterogeneous population using phage display. / Sørensen, Morten Dræby; Kristensen, Peter.

I: Nature Protocols (Print), Bind 6, Nr. 4, 01.04.2011, s. 509-522.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Sørensen, Morten Dræby ; Kristensen, Peter. / Selection of antibodies against a single rare cell present in a heterogeneous population using phage display. I: Nature Protocols (Print). 2011 ; Bind 6, Nr. 4. s. 509-522.

Bibtex

@article{85ad2a5ac1484441a4900a9d0d84e296,
title = "Selection of antibodies against a single rare cell present in a heterogeneous population using phage display",
abstract = "Here we describe a new method applying phage-displayed antibody libraries to the selection of antibodies against a single identified cell on a glass slide. This is the only described method that has successfully achieved selection of antibodies against a single rare cell in a heterogeneous population of cells. The phage library is incubated with the slide containing the identified rare cell of interest; incubation is followed by UV irradiation while protecting the target cell with a minute disc. The UV light inactivates all phages outside the shielded area by cross-linking the DNA constituting their genomes. The expected yield is between one and ten phage particles from a single cell selection. The encoded antibodies are subsequently produced monoclonally and tested for specificity. This method can be applied within a week to carry out ten or more individual cell selections. Including subsequent testing of antibody specificity, a specific antibody can be identified within 2 months.",
author = "S{\o}rensen, {Morten Dr{\ae}by} and Peter Kristensen",
year = "2011",
month = "4",
day = "1",
doi = "10.1038/nprot.2011.311",
language = "English",
volume = "6",
pages = "509--522",
journal = "Nature Protocols (Print)",
issn = "1754-2189",
publisher = "Nature Publishing Group",
number = "4",

}

RIS

TY - JOUR

T1 - Selection of antibodies against a single rare cell present in a heterogeneous population using phage display

AU - Sørensen, Morten Dræby

AU - Kristensen, Peter

PY - 2011/4/1

Y1 - 2011/4/1

N2 - Here we describe a new method applying phage-displayed antibody libraries to the selection of antibodies against a single identified cell on a glass slide. This is the only described method that has successfully achieved selection of antibodies against a single rare cell in a heterogeneous population of cells. The phage library is incubated with the slide containing the identified rare cell of interest; incubation is followed by UV irradiation while protecting the target cell with a minute disc. The UV light inactivates all phages outside the shielded area by cross-linking the DNA constituting their genomes. The expected yield is between one and ten phage particles from a single cell selection. The encoded antibodies are subsequently produced monoclonally and tested for specificity. This method can be applied within a week to carry out ten or more individual cell selections. Including subsequent testing of antibody specificity, a specific antibody can be identified within 2 months.

AB - Here we describe a new method applying phage-displayed antibody libraries to the selection of antibodies against a single identified cell on a glass slide. This is the only described method that has successfully achieved selection of antibodies against a single rare cell in a heterogeneous population of cells. The phage library is incubated with the slide containing the identified rare cell of interest; incubation is followed by UV irradiation while protecting the target cell with a minute disc. The UV light inactivates all phages outside the shielded area by cross-linking the DNA constituting their genomes. The expected yield is between one and ten phage particles from a single cell selection. The encoded antibodies are subsequently produced monoclonally and tested for specificity. This method can be applied within a week to carry out ten or more individual cell selections. Including subsequent testing of antibody specificity, a specific antibody can be identified within 2 months.

U2 - 10.1038/nprot.2011.311

DO - 10.1038/nprot.2011.311

M3 - Journal article

C2 - 21455187

VL - 6

SP - 509

EP - 522

JO - Nature Protocols (Print)

JF - Nature Protocols (Print)

SN - 1754-2189

IS - 4

ER -