Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections

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Standard

Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections. / Larsen, Simon Asbjørn; Meldgaard, Theresa; Fridriksdottir, Agla J; Lykkemark, Simon; Poulsen, Pi Camilla; Overgaard, Laura F; Petersen, Helene Bundgaard; Petersen, Ole William; Kristensen, Peter.

I: Immunologic Research, Bind 62, Nr. 3, 12.05.2015, s. 263-272.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Larsen, SA, Meldgaard, T, Fridriksdottir, AJ, Lykkemark, S, Poulsen, PC, Overgaard, LF, Petersen, HB, Petersen, OW & Kristensen, P 2015, 'Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections', Immunologic Research, bind 62, nr. 3, s. 263-272. https://doi.org/10.1007/s12026-015-8657-x

APA

Larsen, S. A., Meldgaard, T., Fridriksdottir, A. J., Lykkemark, S., Poulsen, P. C., Overgaard, L. F., Petersen, H. B., Petersen, O. W., & Kristensen, P. (2015). Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections. Immunologic Research, 62(3), 263-272. https://doi.org/10.1007/s12026-015-8657-x

CBE

Larsen SA, Meldgaard T, Fridriksdottir AJ, Lykkemark S, Poulsen PC, Overgaard LF, Petersen HB, Petersen OW, Kristensen P. 2015. Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections. Immunologic Research. 62(3):263-272. https://doi.org/10.1007/s12026-015-8657-x

MLA

Vancouver

Larsen SA, Meldgaard T, Fridriksdottir AJ, Lykkemark S, Poulsen PC, Overgaard LF o.a. Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections. Immunologic Research. 2015 maj 12;62(3):263-272. https://doi.org/10.1007/s12026-015-8657-x

Author

Larsen, Simon Asbjørn ; Meldgaard, Theresa ; Fridriksdottir, Agla J ; Lykkemark, Simon ; Poulsen, Pi Camilla ; Overgaard, Laura F ; Petersen, Helene Bundgaard ; Petersen, Ole William ; Kristensen, Peter. / Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections. I: Immunologic Research. 2015 ; Bind 62, Nr. 3. s. 263-272.

Bibtex

@article{c4748ea4fbc3478cb06a7ce8d507da26,
title = "Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections",
abstract = "Breast cancer tumors are composed of heterogeneous cell populations. These populations display a high variance in morphology, growth and metastatic propensity. They respond differently to therapeutic interventions, and some may be more prone to cause recurrence. Studying individual subpopulations of breast cancer may provide crucial knowledge for the development of individualized therapy. However, this process is challenged by the availability of biomarkers able to identify subpopulations specifically. Here, we demonstrate an approach for phage display selection of recombinant antibody fragments on cryostat sections of human breast cancer tissue. This method allows for selection of recombinant antibodies binding to antigens specifically expressed in a small part of the tissue section. In this case, a CD271(+) subpopulation of breast cancer cells was targeted, and these may be potential breast cancer stem cells. We isolated an antibody fragment LH 7, which in immunohistochemistry experiments demonstrates specific binding to breast cancer subpopulations. The selection of antibody fragments directly on small defined areas within a larger section of malignant tissue is a novel approach by which it is possible to better target cellular heterogeneity in proteomic studies. The identification of novel biomarkers is relevant for our understanding and intervention in human diseases. The selection of the breast cancer-specific antibody fragment LH 7 may reveal novel subpopulation-specific biomarkers, which has the potential to provide new insight and treatment strategies for breast cancer.",
author = "Larsen, {Simon Asbj{\o}rn} and Theresa Meldgaard and Fridriksdottir, {Agla J} and Simon Lykkemark and Poulsen, {Pi Camilla} and Overgaard, {Laura F} and Petersen, {Helene Bundgaard} and Petersen, {Ole William} and Peter Kristensen",
year = "2015",
month = may,
day = "12",
doi = "10.1007/s12026-015-8657-x",
language = "English",
volume = "62",
pages = "263--272",
journal = "Immunologic Research",
issn = "0257-277X",
publisher = "Humana Press, Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Selection of a breast cancer subpopulation-specific antibody using phage display on tissue sections

AU - Larsen, Simon Asbjørn

AU - Meldgaard, Theresa

AU - Fridriksdottir, Agla J

AU - Lykkemark, Simon

AU - Poulsen, Pi Camilla

AU - Overgaard, Laura F

AU - Petersen, Helene Bundgaard

AU - Petersen, Ole William

AU - Kristensen, Peter

PY - 2015/5/12

Y1 - 2015/5/12

N2 - Breast cancer tumors are composed of heterogeneous cell populations. These populations display a high variance in morphology, growth and metastatic propensity. They respond differently to therapeutic interventions, and some may be more prone to cause recurrence. Studying individual subpopulations of breast cancer may provide crucial knowledge for the development of individualized therapy. However, this process is challenged by the availability of biomarkers able to identify subpopulations specifically. Here, we demonstrate an approach for phage display selection of recombinant antibody fragments on cryostat sections of human breast cancer tissue. This method allows for selection of recombinant antibodies binding to antigens specifically expressed in a small part of the tissue section. In this case, a CD271(+) subpopulation of breast cancer cells was targeted, and these may be potential breast cancer stem cells. We isolated an antibody fragment LH 7, which in immunohistochemistry experiments demonstrates specific binding to breast cancer subpopulations. The selection of antibody fragments directly on small defined areas within a larger section of malignant tissue is a novel approach by which it is possible to better target cellular heterogeneity in proteomic studies. The identification of novel biomarkers is relevant for our understanding and intervention in human diseases. The selection of the breast cancer-specific antibody fragment LH 7 may reveal novel subpopulation-specific biomarkers, which has the potential to provide new insight and treatment strategies for breast cancer.

AB - Breast cancer tumors are composed of heterogeneous cell populations. These populations display a high variance in morphology, growth and metastatic propensity. They respond differently to therapeutic interventions, and some may be more prone to cause recurrence. Studying individual subpopulations of breast cancer may provide crucial knowledge for the development of individualized therapy. However, this process is challenged by the availability of biomarkers able to identify subpopulations specifically. Here, we demonstrate an approach for phage display selection of recombinant antibody fragments on cryostat sections of human breast cancer tissue. This method allows for selection of recombinant antibodies binding to antigens specifically expressed in a small part of the tissue section. In this case, a CD271(+) subpopulation of breast cancer cells was targeted, and these may be potential breast cancer stem cells. We isolated an antibody fragment LH 7, which in immunohistochemistry experiments demonstrates specific binding to breast cancer subpopulations. The selection of antibody fragments directly on small defined areas within a larger section of malignant tissue is a novel approach by which it is possible to better target cellular heterogeneity in proteomic studies. The identification of novel biomarkers is relevant for our understanding and intervention in human diseases. The selection of the breast cancer-specific antibody fragment LH 7 may reveal novel subpopulation-specific biomarkers, which has the potential to provide new insight and treatment strategies for breast cancer.

U2 - 10.1007/s12026-015-8657-x

DO - 10.1007/s12026-015-8657-x

M3 - Journal article

C2 - 25963139

VL - 62

SP - 263

EP - 272

JO - Immunologic Research

JF - Immunologic Research

SN - 0257-277X

IS - 3

ER -