Secretagogin is a new neuroendocrine marker in the human prostate

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Secretagogin is a new neuroendocrine marker in the human prostate. / Adolf, Katja; Wagner, Ludwig; Bergh, Anders et al.

I: Prostate, Bind 67, Nr. 5, 2007, s. 472-484.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Adolf, K, Wagner, L, Bergh, A, Stattin, P, Ottosen, P, Borre, M, Birkenkamp-Demtröder, K, Ørntoft, TF & Tørring, N 2007, 'Secretagogin is a new neuroendocrine marker in the human prostate', Prostate, bind 67, nr. 5, s. 472-484. https://doi.org/10.1002/pros.20523

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Adolf K, Wagner L, Bergh A, Stattin P, Ottosen P, Borre M et al. Secretagogin is a new neuroendocrine marker in the human prostate. Prostate. 2007;67(5):472-484. doi: 10.1002/pros.20523

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Adolf, Katja ; Wagner, Ludwig ; Bergh, Anders et al. / Secretagogin is a new neuroendocrine marker in the human prostate. I: Prostate. 2007 ; Bind 67, Nr. 5. s. 472-484.

Bibtex

@article{f6d7df60102111dcbee902004c4f4f50,
title = "Secretagogin is a new neuroendocrine marker in the human prostate",
abstract = "BACKGROUND: Neuroendocrine (NE) differentiation in prostate cancer (PCa), promoted by NE cell secreted products, appears to be associated with tumor progression, poor prognosis, and hormone-refractory disease. We recently reported secretagogin, a hexa-EF-hand Ca(2+) binding protein, as a novel NE marker in carcinoid tumors of the lung and the gastrointestinal tract. The present study analyzes the expression of secretagogin in normal and malign prostate tissue. METHODS: We analyzed immunoreactivity for secretagogin, chromogranin A (CgA), neuron specific enolase (NSE), and synaptophysin (SYN) in consecutive sections from 87 formalin-fixed paraffin-embedded (FFPE) benign hyperplastic (n = 10) and prostate adenocarcinoma (n = 77) specimens. The intracellular distribution of secretagogin, CgA, and NSE was examined by confocal fluorescent microscopy, and we characterized secretagogin in eight samples by Western blotting. RESULTS: Secretagogin is cytoplasmic and nuclear expressed in NE and NE differentiated cells, and to a lesser extent in epithelial cells, in the benign prostate and prostate adenocarcinoma cells. Secretagogin stained 82% (46/56) of benign and 71% (48/68) of prostate adenocarcinomas and co-localized with the NE markers CgA and NSE. The expression of secretagogin is significantly correlated to CgA (P < 0.001) and NSE (P < 0.048) in prostate adenocarcinoma and to CgA in normal epithelium (P < 0.028). CONCLUSIONS: Secretagogin is a novel NE marker in the prostate with more extended immunoreactivity compared to the NE markers CgA, SYN, and NSE. Secretagogin is widely expressed in prostatic adenocarcinoma as opposed to adenocarcinomas in other organs.",
author = "Katja Adolf and Ludwig Wagner and Anders Bergh and P{\"a}r Stattin and Peter Ottosen and Michael Borre and Karin Birkenkamp-Demtr{\"o}der and {\O}rntoft, {Torben Falck} and Niels T{\o}rring",
year = "2007",
doi = "10.1002/pros.20523",
language = "English",
volume = "67",
pages = "472--484",
journal = "Prostate",
issn = "0270-4137",
publisher = "JohnWiley & Sons, Inc.",
number = "5",

}

RIS

TY - JOUR

T1 - Secretagogin is a new neuroendocrine marker in the human prostate

AU - Adolf, Katja

AU - Wagner, Ludwig

AU - Bergh, Anders

AU - Stattin, Pär

AU - Ottosen, Peter

AU - Borre, Michael

AU - Birkenkamp-Demtröder, Karin

AU - Ørntoft, Torben Falck

AU - Tørring, Niels

PY - 2007

Y1 - 2007

N2 - BACKGROUND: Neuroendocrine (NE) differentiation in prostate cancer (PCa), promoted by NE cell secreted products, appears to be associated with tumor progression, poor prognosis, and hormone-refractory disease. We recently reported secretagogin, a hexa-EF-hand Ca(2+) binding protein, as a novel NE marker in carcinoid tumors of the lung and the gastrointestinal tract. The present study analyzes the expression of secretagogin in normal and malign prostate tissue. METHODS: We analyzed immunoreactivity for secretagogin, chromogranin A (CgA), neuron specific enolase (NSE), and synaptophysin (SYN) in consecutive sections from 87 formalin-fixed paraffin-embedded (FFPE) benign hyperplastic (n = 10) and prostate adenocarcinoma (n = 77) specimens. The intracellular distribution of secretagogin, CgA, and NSE was examined by confocal fluorescent microscopy, and we characterized secretagogin in eight samples by Western blotting. RESULTS: Secretagogin is cytoplasmic and nuclear expressed in NE and NE differentiated cells, and to a lesser extent in epithelial cells, in the benign prostate and prostate adenocarcinoma cells. Secretagogin stained 82% (46/56) of benign and 71% (48/68) of prostate adenocarcinomas and co-localized with the NE markers CgA and NSE. The expression of secretagogin is significantly correlated to CgA (P < 0.001) and NSE (P < 0.048) in prostate adenocarcinoma and to CgA in normal epithelium (P < 0.028). CONCLUSIONS: Secretagogin is a novel NE marker in the prostate with more extended immunoreactivity compared to the NE markers CgA, SYN, and NSE. Secretagogin is widely expressed in prostatic adenocarcinoma as opposed to adenocarcinomas in other organs.

AB - BACKGROUND: Neuroendocrine (NE) differentiation in prostate cancer (PCa), promoted by NE cell secreted products, appears to be associated with tumor progression, poor prognosis, and hormone-refractory disease. We recently reported secretagogin, a hexa-EF-hand Ca(2+) binding protein, as a novel NE marker in carcinoid tumors of the lung and the gastrointestinal tract. The present study analyzes the expression of secretagogin in normal and malign prostate tissue. METHODS: We analyzed immunoreactivity for secretagogin, chromogranin A (CgA), neuron specific enolase (NSE), and synaptophysin (SYN) in consecutive sections from 87 formalin-fixed paraffin-embedded (FFPE) benign hyperplastic (n = 10) and prostate adenocarcinoma (n = 77) specimens. The intracellular distribution of secretagogin, CgA, and NSE was examined by confocal fluorescent microscopy, and we characterized secretagogin in eight samples by Western blotting. RESULTS: Secretagogin is cytoplasmic and nuclear expressed in NE and NE differentiated cells, and to a lesser extent in epithelial cells, in the benign prostate and prostate adenocarcinoma cells. Secretagogin stained 82% (46/56) of benign and 71% (48/68) of prostate adenocarcinomas and co-localized with the NE markers CgA and NSE. The expression of secretagogin is significantly correlated to CgA (P < 0.001) and NSE (P < 0.048) in prostate adenocarcinoma and to CgA in normal epithelium (P < 0.028). CONCLUSIONS: Secretagogin is a novel NE marker in the prostate with more extended immunoreactivity compared to the NE markers CgA, SYN, and NSE. Secretagogin is widely expressed in prostatic adenocarcinoma as opposed to adenocarcinomas in other organs.

U2 - 10.1002/pros.20523

DO - 10.1002/pros.20523

M3 - Journal article

C2 - 17285592

VL - 67

SP - 472

EP - 484

JO - Prostate

JF - Prostate

SN - 0270-4137

IS - 5

ER -