Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

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Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration. / Cisek, Richard; Tokarz, Danielle; Steup, Martin; Tetlow, Ian J; Emes, Michael J; Hebelstrup, Kim; Blennow, Andreas; Barzda, Virginijus.

I: Biomedical Optics Express, Bind 6, Nr. 10, 01.09.2015, s. 3694-3700.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Cisek, R, Tokarz, D, Steup, M, Tetlow, IJ, Emes, MJ, Hebelstrup, K, Blennow, A & Barzda, V 2015, 'Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration' Biomedical Optics Express, bind 6, nr. 10, s. 3694-3700. https://doi.org/10.1364/BOE.6.003694

APA

Cisek, R., Tokarz, D., Steup, M., Tetlow, I. J., Emes, M. J., Hebelstrup, K., ... Barzda, V. (2015). Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration. Biomedical Optics Express, 6(10), 3694-3700. https://doi.org/10.1364/BOE.6.003694

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Author

Cisek, Richard ; Tokarz, Danielle ; Steup, Martin ; Tetlow, Ian J ; Emes, Michael J ; Hebelstrup, Kim ; Blennow, Andreas ; Barzda, Virginijus. / Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration. I: Biomedical Optics Express. 2015 ; Bind 6, Nr. 10. s. 3694-3700.

Bibtex

@article{3c55d0aa43fb49b8863b06e149e78a00,
title = "Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration",
abstract = "Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R’SHG) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R’SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.",
author = "Richard Cisek and Danielle Tokarz and Martin Steup and Tetlow, {Ian J} and Emes, {Michael J} and Kim Hebelstrup and Andreas Blennow and Virginijus Barzda",
year = "2015",
month = "9",
day = "1",
doi = "10.1364/BOE.6.003694",
language = "English",
volume = "6",
pages = "3694--3700",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "Optical Society of America",
number = "10",

}

RIS

TY - JOUR

T1 - Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

AU - Cisek, Richard

AU - Tokarz, Danielle

AU - Steup, Martin

AU - Tetlow, Ian J

AU - Emes, Michael J

AU - Hebelstrup, Kim

AU - Blennow, Andreas

AU - Barzda, Virginijus

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R’SHG) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R’SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.

AB - Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R’SHG) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R’SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.

U2 - 10.1364/BOE.6.003694

DO - 10.1364/BOE.6.003694

M3 - Journal article

VL - 6

SP - 3694

EP - 3700

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 10

ER -