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Scalable preparation of high purity rutin fatty acid esters following enzymatic synthesis

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  • Molekylærbiologisk Institut
  • Center for Tekniske Kandidatuddannelser
  • Interdisciplinary Nanoscience Center
  • Institut for Kemi
Investigations into expanded uses of modified flavonoids are often limited by the availability of these high purity compounds. As such, a simple, effective and relatively fast method for isolation of gram quantities of both long and medium chain fatty acid esters of rutin following scaled-up biosynthesis reactions was established. Acylation reactions of rutin and palmitic or lauric acids were efficient in systems containing dried acetone and molecular sieves, yielding from 70–77% bioconversion after 96 h. Thereafter, high purity isolates (>97%) were easily obtained in significant quantities following a two-step solvent purification procedure whereby excess fatty acid substrate was first removed in a heptane/water (4:1, v/v) system, followed by selective ester extraction using an ethyl acetate/water system (1:6, v/v) at elevated temperature.
TidsskriftJournal of the American Oil Chemists' Society
Sider (fra-til)55-61
StatusUdgivet - 2010

Bibliografisk note

Paper id:: DOI 10.1007/s11746-009-1471-7

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