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Satellite cells sourced from bull calves and dairy cows differs in proliferative and myogenic capacity – Implications for cultivated meat

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Cultivated meat produced with primary muscle satellite cells (SCs) will need a continuous supply of isolated cell material from relevant animal donors. Factors such as age, sex, and breed, along with the sustainability and availability of donor animals, could determine the most appropriate donor type for an efficient production. In this study, we focus on the proliferation and differentiation of bovine SCs isolated from bull calf and dairy cow muscle samples. The proliferative performance of bull calf SCs was significantly better than SCs from dairy cows, however a dynamic differentiation assay revealed that the degree of fusion and formation of myotubes were similar between donor types. Furthermore, the proliferation of SCs from both donor types was enhanced using an in-house developed serum-free media compared to 10% FBS, which also delayed myogenic differentiation and increased final cell population density. Using gene chip transcriptomics, we identified several differentially expressed genes between the two donor types, which could help explain the observed cellular differences. This data also revealed a high biological variance between the three replicate animals within donor type, which seemed to be decreased when using our in-house serum-free media. With the use of the powerful imaging modalities of Cytation 5, we developed a novel high contrast brightfield-enabled label-free myotube quantification method along with a more efficient end-point fusion analysis using Phalloidin-staining. The results give new insights into the bovine SC biology and potential use of bull calves and dairy cows as relevant donor animals for cultivated beef cell sourcing. The newly developed differentiation assays will further enhance future research within the field of cultivated meat and SC biology.

TidsskriftFood Research International
NummerPart 1
StatusUdgivet - nov. 2023

Bibliografisk note

Funding Information:
The support from the slaughterhouse Danish Crown, Aalborg, Denmark and the Department of Animal and Veterinary Sciences, Aarhus University, Denmark during sampling of muscles and the technical assistance from Jens Askov Jensen, Department of Food Science, Aarhus University, Denmark for sampling and isolation of satellite cells is highly appreciated. Valuable support from Dang Quang Svend Le was received with the design and production of multiple sampling devices to reduce the contamination risks during SC isolation.

Funding Information:
The research was supported by the Danish Agricultural Agency (33010-NIFA-19–716), Ministry of Food, Agriculture and Fisheries of Denmark, The Graduate School of Technical Sciences (GSTS), Aarhus University and the Center of Innovative Food Research (CiFOOD), Aarhus University, Denmark. Data were generated though accessing research infrastructure at Aarhus University, including FOODHAY (Food and Health Open Innovation Laboratory, Danish Roadmap for Research Infrastructure).

Publisher Copyright:
© 2023 The Authors

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