Aarhus University Seal / Aarhus Universitets segl

Rye bran modified with cell wall-degrading enzymes influences the kinetics of plant lignans but not of enterolignans in multicatheterized pigs

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Rye bran modified with cell wall-degrading enzymes influences the kinetics of plant lignans but not of enterolignans in multicatheterized pigs. / Bolvig, Anne Katrine; Nørskov, Natalja; van Vliet, Sophie; Foldager, Leslie; Curtasu, Mihai Victor ; Hedemann, Mette Skou; Sørensen, Jens F; Lærke, Helle Nygaard; Knudsen, Knud Erik Bach.

I: Journal of Nutrition, Bind 147, Nr. 12, 01.12.2017, s. 2220-2227 .

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{f28479792e0b442fbac14127f0681919,
title = "Rye bran modified with cell wall-degrading enzymes influences the kinetics of plant lignans but not of enterolignans in multicatheterized pigs",
abstract = "Background: Whole-grain intake is associated with a lower risk of chronic Western-style diseases, possibly brought about by the high concentration of phytochemicals, among them plant lignans (PLs), in the grains. Objective: We studied whether treatment of rye bran with cell wall–degrading enzymes changed the solubility and kinetics of PLs in multicatheterized pigs. Methods: Ten female Duroc × Danish Landrace × Yorkshire pigs (60.3 ± 2.3 kg at surgery) fitted with permanent catheters were included in an incomplete crossover study. The pigs were fed 2 experimental diets for 1–7 d. The diets were rich in PLs and based on nontreated lignan-rich (LR) [lignan concentration: 20.2 mg dry matter (DM)/kg] or enzymatically treated lignan-rich (ENZLR, lignan concentration: 27.8 mg DM/kg) rye bran. Plasma concentrations of PLs and enterolignans were quantified with the use of targeted LC-tandem mass spectrometry. Data were log transformed and analyzed with mixed-effects, 1-compartment, and asymptotic regression models. Results: The availability of PLs was 38% greater in ENZLR than in LR, and the soluble fraction of PLs was 49% in ENZLR compared with 35% in LR diets. PLs appeared in the circulation 30 min after intake of both the ENZLR and LR diets. Postprandially, consumption of ENZLR resulted in a 4-times-greater (P < 0.0001) plasma PL concentration compared with LR. The area under the curve (AUC) measured 0–360 min after ENZLR intake was ∼2 times higher than after LR intake. A 1-compartment model could describe the postprandial increase in plasma concentration after ENZLR intake, whereas an asymptotic regression model described the plasma concentrations after LR intake. Despite increased available and soluble PLs, ENZLR did not increase plasma enterolignans. Conclusion: The modification of rye bran with cell wall–degrading enzymes resulted in significantly greater plasma concentrations of PLs and the 4-h AUC, particularly syringaresinol, in multicatheterized pigs. ",
keywords = "lignans, cell wall-degrading enzymes, kinetics, catherized pigs, syringaresinol",
author = "Bolvig, {Anne Katrine} and Natalja N{\o}rskov and {van Vliet}, Sophie and Leslie Foldager and Curtasu, {Mihai Victor} and Hedemann, {Mette Skou} and S{\o}rensen, {Jens F} and L{\ae}rke, {Helle Nygaard} and Knudsen, {Knud Erik Bach}",
year = "2017",
month = dec,
day = "1",
doi = "10.3945/jn.117.258483",
language = "English",
volume = "147",
pages = "2220--2227 ",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "AMER SOC NUTRITION-ASN",
number = "12",

}

RIS

TY - JOUR

T1 - Rye bran modified with cell wall-degrading enzymes influences the kinetics of plant lignans but not of enterolignans in multicatheterized pigs

AU - Bolvig, Anne Katrine

AU - Nørskov, Natalja

AU - van Vliet, Sophie

AU - Foldager, Leslie

AU - Curtasu, Mihai Victor

AU - Hedemann, Mette Skou

AU - Sørensen, Jens F

AU - Lærke, Helle Nygaard

AU - Knudsen, Knud Erik Bach

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Background: Whole-grain intake is associated with a lower risk of chronic Western-style diseases, possibly brought about by the high concentration of phytochemicals, among them plant lignans (PLs), in the grains. Objective: We studied whether treatment of rye bran with cell wall–degrading enzymes changed the solubility and kinetics of PLs in multicatheterized pigs. Methods: Ten female Duroc × Danish Landrace × Yorkshire pigs (60.3 ± 2.3 kg at surgery) fitted with permanent catheters were included in an incomplete crossover study. The pigs were fed 2 experimental diets for 1–7 d. The diets were rich in PLs and based on nontreated lignan-rich (LR) [lignan concentration: 20.2 mg dry matter (DM)/kg] or enzymatically treated lignan-rich (ENZLR, lignan concentration: 27.8 mg DM/kg) rye bran. Plasma concentrations of PLs and enterolignans were quantified with the use of targeted LC-tandem mass spectrometry. Data were log transformed and analyzed with mixed-effects, 1-compartment, and asymptotic regression models. Results: The availability of PLs was 38% greater in ENZLR than in LR, and the soluble fraction of PLs was 49% in ENZLR compared with 35% in LR diets. PLs appeared in the circulation 30 min after intake of both the ENZLR and LR diets. Postprandially, consumption of ENZLR resulted in a 4-times-greater (P < 0.0001) plasma PL concentration compared with LR. The area under the curve (AUC) measured 0–360 min after ENZLR intake was ∼2 times higher than after LR intake. A 1-compartment model could describe the postprandial increase in plasma concentration after ENZLR intake, whereas an asymptotic regression model described the plasma concentrations after LR intake. Despite increased available and soluble PLs, ENZLR did not increase plasma enterolignans. Conclusion: The modification of rye bran with cell wall–degrading enzymes resulted in significantly greater plasma concentrations of PLs and the 4-h AUC, particularly syringaresinol, in multicatheterized pigs.

AB - Background: Whole-grain intake is associated with a lower risk of chronic Western-style diseases, possibly brought about by the high concentration of phytochemicals, among them plant lignans (PLs), in the grains. Objective: We studied whether treatment of rye bran with cell wall–degrading enzymes changed the solubility and kinetics of PLs in multicatheterized pigs. Methods: Ten female Duroc × Danish Landrace × Yorkshire pigs (60.3 ± 2.3 kg at surgery) fitted with permanent catheters were included in an incomplete crossover study. The pigs were fed 2 experimental diets for 1–7 d. The diets were rich in PLs and based on nontreated lignan-rich (LR) [lignan concentration: 20.2 mg dry matter (DM)/kg] or enzymatically treated lignan-rich (ENZLR, lignan concentration: 27.8 mg DM/kg) rye bran. Plasma concentrations of PLs and enterolignans were quantified with the use of targeted LC-tandem mass spectrometry. Data were log transformed and analyzed with mixed-effects, 1-compartment, and asymptotic regression models. Results: The availability of PLs was 38% greater in ENZLR than in LR, and the soluble fraction of PLs was 49% in ENZLR compared with 35% in LR diets. PLs appeared in the circulation 30 min after intake of both the ENZLR and LR diets. Postprandially, consumption of ENZLR resulted in a 4-times-greater (P < 0.0001) plasma PL concentration compared with LR. The area under the curve (AUC) measured 0–360 min after ENZLR intake was ∼2 times higher than after LR intake. A 1-compartment model could describe the postprandial increase in plasma concentration after ENZLR intake, whereas an asymptotic regression model described the plasma concentrations after LR intake. Despite increased available and soluble PLs, ENZLR did not increase plasma enterolignans. Conclusion: The modification of rye bran with cell wall–degrading enzymes resulted in significantly greater plasma concentrations of PLs and the 4-h AUC, particularly syringaresinol, in multicatheterized pigs.

KW - lignans

KW - cell wall-degrading enzymes

KW - kinetics

KW - catherized pigs

KW - syringaresinol

U2 - 10.3945/jn.117.258483

DO - 10.3945/jn.117.258483

M3 - Journal article

C2 - 28978677

VL - 147

SP - 2220

EP - 2227

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 12

ER -