Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

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Standard

Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities. / Zgadzaj, Rafal; Garrido-Oter, Ruben; Jensen, Dorthe Bodker; Koprivova, Anna; Schulze-Lefert, Paul; Radutoiu, Simona.

I: Proceedings of the National Academy of Sciences of the United States of America, Bind 113, Nr. 49, 06.12.2016, s. E7996-E8005.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Zgadzaj, R, Garrido-Oter, R, Jensen, DB, Koprivova, A, Schulze-Lefert, P & Radutoiu, S 2016, 'Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities', Proceedings of the National Academy of Sciences of the United States of America, bind 113, nr. 49, s. E7996-E8005. https://doi.org/10.1073/pnas.1616564113

APA

Zgadzaj, R., Garrido-Oter, R., Jensen, D. B., Koprivova, A., Schulze-Lefert, P., & Radutoiu, S. (2016). Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities. Proceedings of the National Academy of Sciences of the United States of America, 113(49), E7996-E8005. https://doi.org/10.1073/pnas.1616564113

CBE

Zgadzaj R, Garrido-Oter R, Jensen DB, Koprivova A, Schulze-Lefert P, Radutoiu S. 2016. Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities. Proceedings of the National Academy of Sciences of the United States of America. 113(49):E7996-E8005. https://doi.org/10.1073/pnas.1616564113

MLA

Zgadzaj, Rafal o.a.. "Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities". Proceedings of the National Academy of Sciences of the United States of America. 2016, 113(49). E7996-E8005. https://doi.org/10.1073/pnas.1616564113

Vancouver

Zgadzaj R, Garrido-Oter R, Jensen DB, Koprivova A, Schulze-Lefert P, Radutoiu S. Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities. Proceedings of the National Academy of Sciences of the United States of America. 2016 dec 6;113(49):E7996-E8005. https://doi.org/10.1073/pnas.1616564113

Author

Zgadzaj, Rafal ; Garrido-Oter, Ruben ; Jensen, Dorthe Bodker ; Koprivova, Anna ; Schulze-Lefert, Paul ; Radutoiu, Simona. / Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities. I: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Bind 113, Nr. 49. s. E7996-E8005.

Bibtex

@article{4531eaed670e48d79fb9646dc3c38d4c,
title = "Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities",
abstract = "Lotus japonicus has been used for decades as a model legume to study the establishment of binary symbiotic relationships with nitrogen-fixing rhizobia that trigger root nodule organogenesis for bacterial accommodation. Using community profiling of 16S rRNA gene amplicons, we reveal that in Lotus, distinctive noduleand root-inhabiting communities are established by parallel, rather than consecutive, selection of bacteria from the rhizosphere and root compartments. Comparative analyses of wild-type (WT) and symbiotic mutants in Nod factor receptor5 (nfr5), Nodule inception (nin) and Lotus histidine kinase1 (lhk1) genes identified a previously unsuspected role of the nodulation pathway in the establishment of different bacterial assemblages in the root and rhizosphere. We found that the loss of nitrogen-fixing symbiosis dramatically alters community structure in the latter two compartments, affecting at least 14 bacterial orders. The differential plant growth phenotypes seen between WT and the symbiotic mutants in nonsupplemented soil were retained under nitrogen-supplemented conditions that blocked the formation of functional nodules in WT, whereas the symbiosis-impaired mutants maintain an altered community structure in the nitrogen-supplemented soil. This finding provides strong evidence that the root-associated community shift in the symbiotic mutants is a direct consequence of the disabled symbiosis pathway rather than an indirect effect resulting from abolished symbiotic nitrogen fixation. Our findings imply a role of the legume host in selecting a broad taxonomic range of root-associated bacteria that, in addition to rhizobia, likely contribute to plant growth and ecological performance.",
keywords = "Lotus japonicus, microbiota, symbiosis, 16S, nitrogen fixation, RECEPTOR-LIKE KINASE, NITROGEN-FIXATION, SOYBEAN RHIZOSPHERE, RHIZOBIUM-MELILOTI, FUNGAL SYMBIOSIS, MODEL LEGUME, PLANT, MICROBIOTA, NODULATION, EXPRESSION",
author = "Rafal Zgadzaj and Ruben Garrido-Oter and Jensen, {Dorthe Bodker} and Anna Koprivova and Paul Schulze-Lefert and Simona Radutoiu",
year = "2016",
month = dec,
day = "6",
doi = "10.1073/pnas.1616564113",
language = "English",
volume = "113",
pages = "E7996--E8005",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "49",

}

RIS

TY - JOUR

T1 - Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

AU - Zgadzaj, Rafal

AU - Garrido-Oter, Ruben

AU - Jensen, Dorthe Bodker

AU - Koprivova, Anna

AU - Schulze-Lefert, Paul

AU - Radutoiu, Simona

PY - 2016/12/6

Y1 - 2016/12/6

N2 - Lotus japonicus has been used for decades as a model legume to study the establishment of binary symbiotic relationships with nitrogen-fixing rhizobia that trigger root nodule organogenesis for bacterial accommodation. Using community profiling of 16S rRNA gene amplicons, we reveal that in Lotus, distinctive noduleand root-inhabiting communities are established by parallel, rather than consecutive, selection of bacteria from the rhizosphere and root compartments. Comparative analyses of wild-type (WT) and symbiotic mutants in Nod factor receptor5 (nfr5), Nodule inception (nin) and Lotus histidine kinase1 (lhk1) genes identified a previously unsuspected role of the nodulation pathway in the establishment of different bacterial assemblages in the root and rhizosphere. We found that the loss of nitrogen-fixing symbiosis dramatically alters community structure in the latter two compartments, affecting at least 14 bacterial orders. The differential plant growth phenotypes seen between WT and the symbiotic mutants in nonsupplemented soil were retained under nitrogen-supplemented conditions that blocked the formation of functional nodules in WT, whereas the symbiosis-impaired mutants maintain an altered community structure in the nitrogen-supplemented soil. This finding provides strong evidence that the root-associated community shift in the symbiotic mutants is a direct consequence of the disabled symbiosis pathway rather than an indirect effect resulting from abolished symbiotic nitrogen fixation. Our findings imply a role of the legume host in selecting a broad taxonomic range of root-associated bacteria that, in addition to rhizobia, likely contribute to plant growth and ecological performance.

AB - Lotus japonicus has been used for decades as a model legume to study the establishment of binary symbiotic relationships with nitrogen-fixing rhizobia that trigger root nodule organogenesis for bacterial accommodation. Using community profiling of 16S rRNA gene amplicons, we reveal that in Lotus, distinctive noduleand root-inhabiting communities are established by parallel, rather than consecutive, selection of bacteria from the rhizosphere and root compartments. Comparative analyses of wild-type (WT) and symbiotic mutants in Nod factor receptor5 (nfr5), Nodule inception (nin) and Lotus histidine kinase1 (lhk1) genes identified a previously unsuspected role of the nodulation pathway in the establishment of different bacterial assemblages in the root and rhizosphere. We found that the loss of nitrogen-fixing symbiosis dramatically alters community structure in the latter two compartments, affecting at least 14 bacterial orders. The differential plant growth phenotypes seen between WT and the symbiotic mutants in nonsupplemented soil were retained under nitrogen-supplemented conditions that blocked the formation of functional nodules in WT, whereas the symbiosis-impaired mutants maintain an altered community structure in the nitrogen-supplemented soil. This finding provides strong evidence that the root-associated community shift in the symbiotic mutants is a direct consequence of the disabled symbiosis pathway rather than an indirect effect resulting from abolished symbiotic nitrogen fixation. Our findings imply a role of the legume host in selecting a broad taxonomic range of root-associated bacteria that, in addition to rhizobia, likely contribute to plant growth and ecological performance.

KW - Lotus japonicus

KW - microbiota

KW - symbiosis

KW - 16S

KW - nitrogen fixation

KW - RECEPTOR-LIKE KINASE

KW - NITROGEN-FIXATION

KW - SOYBEAN RHIZOSPHERE

KW - RHIZOBIUM-MELILOTI

KW - FUNGAL SYMBIOSIS

KW - MODEL LEGUME

KW - PLANT

KW - MICROBIOTA

KW - NODULATION

KW - EXPRESSION

U2 - 10.1073/pnas.1616564113

DO - 10.1073/pnas.1616564113

M3 - Journal article

C2 - 27864511

VL - 113

SP - E7996-E8005

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 49

ER -