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Role of short chain fatty acids to counteract inflammatory stress in mucus secreting HT29-MTX cells
Publikation: Bidrag til bog/antologi/rapport/proceeding › Konferenceabstrakt i proceedings › Forskning › peer review
DOI
- https://doi.org/10.3920/978-90-8686-918-3
Forlagets udgivne version
- C. Giromini, Italien
- A. Baldi, Italien
- R Rebucci, Italien
- T. S. Sundaram, Italien
- Stig Purup
The integrity of the gastrointestinal barrier represents the first step in maintaining the gut health, which affects production animals’ performance and welfare. Although SCFAs, especially butyrate, are known to promote intestinalhealth, their role in the protection of the intestinal barrier integrity and function is poorly characterized. The main aim of the study was to set-up an in vitro model of colonic epithelium to study the role of SCFAs as protective nutrientmetabolites for the intestinal epithelial cells exposed to a stress condition. Intestinal goblet HT29-MTX-E12 (E12) cells were differentiated and further stressed with Dextran Sodium Sulphate (DSS), to simulate intestinal inflammation. The effect of butyrate alone (BUT) and the SCFAs mixture (MIX) was tested on intestinal cell viability (LDH test), epithelial integrity (TEER) and permeability (FITC) of differentiated E12 cells exposed to inflammatory stresscondition. MUC2 and MUC5AC gene expression modulation was also evaluated by RT-PCR. Results showed that the concentration of 10% DSS (24 hours) decreased the TEER about 50% compared to control (0% FCS). Treatmentwith a concentration of 10 mM of MIX for 1 and 24 hours significantly (P<0.05) counteracted the decrease of TEER. Treatment with a concentration of 10 mM of BUT for 24 hours significantly (P<0.05) counteracted the decrease of TEER induced with DSS. FITC data demonstrated that the treatment with concentrations of 0.1 mM and 10 mM of MIX for 1 hour significantly (P<0.05) reduced the epithelial permeability of HT29-MTX cells stressed with DSS.Finally, the concentration of 10% DSS for 24 hours significantly reduced MUC2 and MUC5AC gene expression, while treatment with 0.1 mM BUT and MIX for 24 hours significantly promoted MUC2 and MUC5AC gene expression (P<0.05). The present study demonstrates the suitability of E12 cells stressed with DSS as an inflammatory bowel diseases model to study the role of bioactive compounds in promoting intestinal health. SCFAs play an essential role in maintaining intestinal health by affecting epithelial integrity and mucus production.
| Originalsprog | Engelsk |
|---|---|
| Titel | Book of Abstracts of the 72nd Annual Meeting of the European Federation of Animal Science |
| Redaktører | E. Strandberg, L. Pinotti, S. Messori, D. Kenny, M. Lee, J.F. Hocquette, V.A.P. Cadavez, S. Millet, R. Evans, T. Veldkamp, M. Pastell, G. Pollott |
| Antal sider | 1 |
| Forlag | Wageningen Academic Publishers |
| Udgivelsesår | aug. 2021 |
| Sider | 182-182 |
| ISBN (trykt) | 978-90-8686-366-2 |
| ISBN (Elektronisk) | 978-90-8686-918-3 |
| DOI | |
| Status | Udgivet - aug. 2021 |
| Begivenhed | 72nd Annual Meeting of the European Federation of Animal Science - Congress Centre Davos, Davos, Schweiz Varighed: 29 aug. 2021 → 3 sep. 2021 |
Konference
| Konference | 72nd Annual Meeting of the European Federation of Animal Science |
|---|---|
| Lokation | Congress Centre Davos |
| Land | Schweiz |
| By | Davos |
| Periode | 29/08/2021 → 03/09/2021 |
| Serietitel | EAAP Book of Abstracts |
|---|---|
| Vol/bind | 27 |
| ISSN | 1382-6077 |
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