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Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells

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Standard

Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells. / Giromini, Carlotta; Baldi, Antonella; Rebucci, Raffaella et al.
I: Foods, Bind 11, Nr. 13, 1983, 07.2022.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Harvard

Giromini, C, Baldi, A, Rebucci, R, Lanzoni, D, Policardi, M, Sundaram, TS & Purup, S 2022, 'Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells', Foods, bind 11, nr. 13, 1983. https://doi.org/10.3390/foods11131983

APA

Giromini, C., Baldi, A., Rebucci, R., Lanzoni, D., Policardi, M., Sundaram, T. S., & Purup, S. (2022). Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells. Foods, 11(13), artikel 1983. https://doi.org/10.3390/foods11131983

CBE

Giromini C, Baldi A, Rebucci R, Lanzoni D, Policardi M, Sundaram TS, Purup S. 2022. Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells. Foods. 11(13):Article 1983. https://doi.org/10.3390/foods11131983

MLA

Giromini, Carlotta et al. "Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells". Foods. 2022. 11(13). https://doi.org/10.3390/foods11131983

Vancouver

Giromini C, Baldi A, Rebucci R, Lanzoni D, Policardi M, Sundaram TS et al. Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells. Foods. 2022 jul.;11(13):1983. doi: 10.3390/foods11131983

Author

Giromini, Carlotta ; Baldi, Antonella ; Rebucci, Raffaella et al. / Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells. I: Foods. 2022 ; Bind 11, Nr. 13.

Bibtex

@article{bb5768df13da4da5b42f0eb42da6254a,
title = "Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells",
abstract = "Short chain fatty acids (SCFAs), especially butyrate (BUT), are known to promote intestinal health, but their role in the protection of intestinal barrier integrity is poorly characterized. The aim of the study was to set up an in vitro model of human colon epithelium using HT29-MTX-E12 cells to delineate the potential role of SCFAs under stress conditions. Accordingly, the HT29-MTX-E12 cells were differentiated for 42 days and subsequently exposed to dextran sulphate sodium (DSS). Further, the effects of BUT or its mixture with acetate and propionate (SCFAs-MIX) were tested to study proliferation, epithelial integrity and mucus production. The results showed that the concentration of 10% DSS for 24 h decreased the TEER about 50% compared to the control in HT29-MTX-E12 cells. The pre-treatment on HT29-MTX-E12 cells with BUT or SCFAs-MIX at specific concentrations significantly (p < 0.05) reduced the DSS-induced damage on epithelial cell integrity and permeability. Further, the treatment with specific concentrations of BUT and SCFAs-MIX for 24 h significantly promoted ZO-1, MUC2 and MUC5AC mRNA expression (p < 0.005). The present study demonstrated the suitability of HT29-MTX-E12 cells treated with DSS as an in vitro stress model of inflammatory bowel disease, which enabled us to understand the effect of bioactive SCFAs on the intestinal barrier.",
keywords = "fatty acids, gut health, dietary fibre, mucus layer",
author = "Carlotta Giromini and Antonella Baldi and Raffaella Rebucci and Davide Lanzoni and Martina Policardi and Sundaram, {Tamil selvi} and Stig Purup",
year = "2022",
month = jul,
doi = "10.3390/foods11131983",
language = "English",
volume = "11",
journal = "Foods",
issn = "2304-8158",
publisher = "MDPI AG",
number = "13",

}

RIS

TY - JOUR

T1 - Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells

AU - Giromini, Carlotta

AU - Baldi, Antonella

AU - Rebucci, Raffaella

AU - Lanzoni, Davide

AU - Policardi, Martina

AU - Sundaram, Tamil selvi

AU - Purup, Stig

PY - 2022/7

Y1 - 2022/7

N2 - Short chain fatty acids (SCFAs), especially butyrate (BUT), are known to promote intestinal health, but their role in the protection of intestinal barrier integrity is poorly characterized. The aim of the study was to set up an in vitro model of human colon epithelium using HT29-MTX-E12 cells to delineate the potential role of SCFAs under stress conditions. Accordingly, the HT29-MTX-E12 cells were differentiated for 42 days and subsequently exposed to dextran sulphate sodium (DSS). Further, the effects of BUT or its mixture with acetate and propionate (SCFAs-MIX) were tested to study proliferation, epithelial integrity and mucus production. The results showed that the concentration of 10% DSS for 24 h decreased the TEER about 50% compared to the control in HT29-MTX-E12 cells. The pre-treatment on HT29-MTX-E12 cells with BUT or SCFAs-MIX at specific concentrations significantly (p < 0.05) reduced the DSS-induced damage on epithelial cell integrity and permeability. Further, the treatment with specific concentrations of BUT and SCFAs-MIX for 24 h significantly promoted ZO-1, MUC2 and MUC5AC mRNA expression (p < 0.005). The present study demonstrated the suitability of HT29-MTX-E12 cells treated with DSS as an in vitro stress model of inflammatory bowel disease, which enabled us to understand the effect of bioactive SCFAs on the intestinal barrier.

AB - Short chain fatty acids (SCFAs), especially butyrate (BUT), are known to promote intestinal health, but their role in the protection of intestinal barrier integrity is poorly characterized. The aim of the study was to set up an in vitro model of human colon epithelium using HT29-MTX-E12 cells to delineate the potential role of SCFAs under stress conditions. Accordingly, the HT29-MTX-E12 cells were differentiated for 42 days and subsequently exposed to dextran sulphate sodium (DSS). Further, the effects of BUT or its mixture with acetate and propionate (SCFAs-MIX) were tested to study proliferation, epithelial integrity and mucus production. The results showed that the concentration of 10% DSS for 24 h decreased the TEER about 50% compared to the control in HT29-MTX-E12 cells. The pre-treatment on HT29-MTX-E12 cells with BUT or SCFAs-MIX at specific concentrations significantly (p < 0.05) reduced the DSS-induced damage on epithelial cell integrity and permeability. Further, the treatment with specific concentrations of BUT and SCFAs-MIX for 24 h significantly promoted ZO-1, MUC2 and MUC5AC mRNA expression (p < 0.005). The present study demonstrated the suitability of HT29-MTX-E12 cells treated with DSS as an in vitro stress model of inflammatory bowel disease, which enabled us to understand the effect of bioactive SCFAs on the intestinal barrier.

KW - fatty acids

KW - gut health

KW - dietary fibre

KW - mucus layer

U2 - 10.3390/foods11131983

DO - 10.3390/foods11131983

M3 - Journal article

C2 - 35804798

VL - 11

JO - Foods

JF - Foods

SN - 2304-8158

IS - 13

M1 - 1983

ER -