TY - JOUR
T1 - RNA Exosome Depletion Reveals Transcription Upstream of Active Human Promoters
AU - Preker, Pascal
AU - Nielsen, Jesper
AU - Kammler, Susanne
AU - Lykke-Andersen, Søren
AU - Christensen, Marianne S
AU - Mapendano, Christophe K
AU - Schierup, Mikkel Heide
AU - Jensen, Torben Heick
PY - 2008/12/19
Y1 - 2008/12/19
N2 - Studies have shown that the bulk of eukaryotic genomes is transcribed. Transcriptome maps are frequently updated, but low-abundant transcripts have likely gone unnoticed. To eliminate RNA degradation, we depleted the exonucleolytic RNA exosome from human cells and then subjected the RNA to tiling microarray analysis. This revealed a class of short, polyadenylated and highly unstable RNAs. These promoter upstream transcripts (PROMPTs) are produced about 0.5 to 2.5 kb upstream of active transcription start sites (TSSs). PROMPT transcription occurs in both sense and antisense directions with respect to the downstream gene. In addition, it requires the presence of the gene promoter, and is positively correlated with gene activity. We propose that PROMPT transcription is a common characteristic of RNA polymerase II (RNAPII) transcribed genes with a possible regulatory potential.
AB - Studies have shown that the bulk of eukaryotic genomes is transcribed. Transcriptome maps are frequently updated, but low-abundant transcripts have likely gone unnoticed. To eliminate RNA degradation, we depleted the exonucleolytic RNA exosome from human cells and then subjected the RNA to tiling microarray analysis. This revealed a class of short, polyadenylated and highly unstable RNAs. These promoter upstream transcripts (PROMPTs) are produced about 0.5 to 2.5 kb upstream of active transcription start sites (TSSs). PROMPT transcription occurs in both sense and antisense directions with respect to the downstream gene. In addition, it requires the presence of the gene promoter, and is positively correlated with gene activity. We propose that PROMPT transcription is a common characteristic of RNA polymerase II (RNAPII) transcribed genes with a possible regulatory potential.
U2 - 10.1126/science.1164096
DO - 10.1126/science.1164096
M3 - Journal article
C2 - 19056938
SN - 0036-8075
VL - 322
SP - 1851
EP - 1854
JO - Science
JF - Science
IS - 5909
ER -