RGD peptide-mediated liposomal curcumin targeted delivery to breast cancer cells

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  • Reza Mahmoudi, Yasuj University of Medical Sciences
  • ,
  • Seyedeh Ashraf Mirahmadi-Babaheidri, Yasuj University of Medical Sciences
  • ,
  • Hamdollah Delaviz, Yasuj University of Medical Sciences
  • ,
  • Mohamad Hassan Fouani, Tarbiat Modares University
  • ,
  • Mohsen Alipour, Jahrom University of Medical Sciences
  • ,
  • Mehrzad Jafari Barmak, Yasuj University of Medical Sciences
  • ,
  • Gunna Christiansen
  • ,
  • Hassan Bardania, Yasuj University of Medical Sciences

In this study, turmeric's active ingredient (Curcumin) was encapsulated into RGD modified Liposomes (RGD-Lip-Cur) its cytotoxic effect on the breast cancer cell line (MCF-7) was evaluated by MTT, flow cytometry and Caspase assay. Liposomes were characterized using transmission electron microscopy (TEM). Results demonstrated that the liposomes were spherical in shape, ranging from 70 to 100 nm. MTT assay revealed that RGD-Lip-Cur had a significant cytotoxic effect on MCF-7 cells at concentrations of 32, 16 and 4 mu g/ml compared to Lip-Cur (P <0.05) and curcumin (P <0.01). The apoptosis assay demonstrated that RGD-Lip-Cur induces the apoptosis in MCF-7 cells (39.6% vs 40.2% for initial and secondary apoptosis) significantly more than Lip-Cur (67.7% vs 9.16% for initial and secondary apoptosis) and free curcumin (7.84% vs 38.8% for initial and secondary apoptosis). Moreover, caspase assay showed that RGD-Lip-Cur activates caspase 3/7 compared to Lip-Cur (P <0.05) and free curcumin (P <0.01). The RGD-Lip-Cur was similar to the control group and had no significant cytotoxicity effect. It is concluded that RGD-Lip-Cur as a novel carrier have high cytotoxicity effect on breast cancer cell line (MCF-7).

OriginalsprogEngelsk
TidsskriftJournal of Biomaterials Applications
Vol/bind35
Nummer7
Sider (fra-til)743-753
ISSN0885-3282
DOI
StatusUdgivet - feb. 2021

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