Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius

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Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius. / Vincze, Éva; Reeves, J M; Lamping, E; Farnden, K J F; Reynolds, P H S.

I: Plant Molecular Biology, Bind 26, 1994, s. 303-311.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Vincze, É, Reeves, JM, Lamping, E, Farnden, KJF & Reynolds, PHS 1994, 'Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius', Plant Molecular Biology, bind 26, s. 303-311. https://doi.org/10.1007/BF00039541

APA

Vincze, É., Reeves, J. M., Lamping, E., Farnden, K. J. F., & Reynolds, P. H. S. (1994). Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius. Plant Molecular Biology, 26, 303-311. https://doi.org/10.1007/BF00039541

CBE

Vincze É, Reeves JM, Lamping E, Farnden KJF, Reynolds PHS. 1994. Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius. Plant Molecular Biology. 26:303-311. https://doi.org/10.1007/BF00039541

MLA

Vancouver

Vincze É, Reeves JM, Lamping E, Farnden KJF, Reynolds PHS. Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius. Plant Molecular Biology. 1994;26:303-311. https://doi.org/10.1007/BF00039541

Author

Vincze, Éva ; Reeves, J M ; Lamping, E ; Farnden, K J F ; Reynolds, P H S. / Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius. I: Plant Molecular Biology. 1994 ; Bind 26. s. 303-311.

Bibtex

@article{535f2d20ac1511dda608000ea68e967b,
title = "Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius",
abstract = "Upon the establishment of an effective nitrogen-fixing symbiosis in amide-transporting plants the enzymatic activity and transcript levels of L-asparaginase are dramatically decreased. This decrease in L-asparaginase activity is essential for the correct functioning of the Rhizobium-legume symbiosis in lupin in which asparagine, synthesized from recently fixed nitrogen, is exported to aerial parts of the plant for use in growth and development. Concomitant with this decrease in L-asparaginase transcript a DNA-binding protein was detected in the nodules. This binding protein was not detectable in ineffective nodules, in nodules treated with nitrate, or in root tips, mature roots, developing flowers or developing seeds. The DNA-binding activity was shown to interact with a 59 bp sequence proximal to the transcription start site. Within this sequence a CTAAAAT direct repeat and a ACTGT/TGTCA incomplete inverted repeat were implicated in the binding of protein to the DNA by DNase I protection experiments. Competitive binding studies with synthesized binding sites were consistent with the CTAAAAT/TGTCA sequence pair proximal to the transcription start site having the highest affinity for the DNA-binding protein. We postulate that this DNA-binding protein is associated with repression of L-asparaginase gene expression in mature lupin root nodules.",
author = "{\'E}va Vincze and Reeves, {J M} and E Lamping and Farnden, {K J F} and Reynolds, {P H S}",
year = "1994",
doi = "10.1007/BF00039541",
language = "English",
volume = "26",
pages = "303--311",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Repression of the L-asparaginase gene during nodule development in Lupinus angustifolius

AU - Vincze, Éva

AU - Reeves, J M

AU - Lamping, E

AU - Farnden, K J F

AU - Reynolds, P H S

PY - 1994

Y1 - 1994

N2 - Upon the establishment of an effective nitrogen-fixing symbiosis in amide-transporting plants the enzymatic activity and transcript levels of L-asparaginase are dramatically decreased. This decrease in L-asparaginase activity is essential for the correct functioning of the Rhizobium-legume symbiosis in lupin in which asparagine, synthesized from recently fixed nitrogen, is exported to aerial parts of the plant for use in growth and development. Concomitant with this decrease in L-asparaginase transcript a DNA-binding protein was detected in the nodules. This binding protein was not detectable in ineffective nodules, in nodules treated with nitrate, or in root tips, mature roots, developing flowers or developing seeds. The DNA-binding activity was shown to interact with a 59 bp sequence proximal to the transcription start site. Within this sequence a CTAAAAT direct repeat and a ACTGT/TGTCA incomplete inverted repeat were implicated in the binding of protein to the DNA by DNase I protection experiments. Competitive binding studies with synthesized binding sites were consistent with the CTAAAAT/TGTCA sequence pair proximal to the transcription start site having the highest affinity for the DNA-binding protein. We postulate that this DNA-binding protein is associated with repression of L-asparaginase gene expression in mature lupin root nodules.

AB - Upon the establishment of an effective nitrogen-fixing symbiosis in amide-transporting plants the enzymatic activity and transcript levels of L-asparaginase are dramatically decreased. This decrease in L-asparaginase activity is essential for the correct functioning of the Rhizobium-legume symbiosis in lupin in which asparagine, synthesized from recently fixed nitrogen, is exported to aerial parts of the plant for use in growth and development. Concomitant with this decrease in L-asparaginase transcript a DNA-binding protein was detected in the nodules. This binding protein was not detectable in ineffective nodules, in nodules treated with nitrate, or in root tips, mature roots, developing flowers or developing seeds. The DNA-binding activity was shown to interact with a 59 bp sequence proximal to the transcription start site. Within this sequence a CTAAAAT direct repeat and a ACTGT/TGTCA incomplete inverted repeat were implicated in the binding of protein to the DNA by DNase I protection experiments. Competitive binding studies with synthesized binding sites were consistent with the CTAAAAT/TGTCA sequence pair proximal to the transcription start site having the highest affinity for the DNA-binding protein. We postulate that this DNA-binding protein is associated with repression of L-asparaginase gene expression in mature lupin root nodules.

U2 - 10.1007/BF00039541

DO - 10.1007/BF00039541

M3 - Journal article

VL - 26

SP - 303

EP - 311

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

ER -