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Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

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  • Scott Maynard, Center for Healthy Aging at the University of Copenhagen, Danmark
  • Guido Keijzers, Center for Healthy Aging at the University of Copenhagen, Danmark
  • Martin Gram, Center for Healthy Aging at the University of Copenhagen, Danmark
  • Claus Desler, Center for Healthy Aging at the University of Copenhagen, Danmark
  • Laila Bendix, Afdeling for Social Medicin, Danmark
  • Esben Budtz-Jørgensen, Department of Public Health, University of Copenhagen, Danmark
  • Drude Molbo, Center for Healthy Aging at the University of Copenhagen, Danmark
  • Deborah L Croteau
  • ,
  • Merete Osler, Danish Aging Research Center, University of Southern Denmark, Danmark
  • Tinna Stevnsner
  • Lene Juel Rasmussen, Center for Healthy Aging at the University of Copenhagen, Danmark
  • Flemming Dela, Center for Healthy Aging at the University of Copenhagen, Danmark
  • Kirsten Avlund, Center for Healthy Aging at the University of Copenhagen, Danmark
  • Vilhelm Bohr, Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Danmark
Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.
OriginalsprogEngelsk
TidsskriftNature Communications
Vol/bind5
Nummer11
Sider (fra-til)850-864
Antal sider15
ISSN2041-1723
StatusUdgivet - 11 nov. 2013

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