Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

Scott Maynard; Guido Keijzers; Martin Gram; Claus Desler; Laila Bendix; Esben Budtz-Jørgensen; Drude Molbo; Deborah L Croteau; Merete Osler; Tinna Stevnsner; Lene Juel Rasmussen; Flemming Dela; Kirsten Avlund; Vilhelm Bohr

Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

Scott Maynard, Guido Keijzers, Martin Gram, Claus Desler, Laila Bendix, Esben Budtz-Jørgensen, Drude Molbo, Deborah L Croteau, Merete Osler, Tinna Stevnsner, Lene Juel Rasmussen, Flemming Dela, Kirsten Avlund, Vilhelm Bohr

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

40 Citationer (Scopus)

Abstract

Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.

Originalsprog	Engelsk
Tidsskrift	Nature Communications
Vol/bind	5
Nummer	11
Sider (fra-til)	850-864
Antal sider	15
ISSN	2041-1723
Status	Udgivet - 11 nov. 2013

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http://www.impactaging.com/papers/v5/n11/abs/100618a.html

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Maynard, S., Keijzers, G., Gram, M., Desler, C., Bendix, L., Budtz-Jørgensen, E., Molbo, D., Croteau, D. L., Osler, M., Stevnsner, T., Rasmussen, L. J., Dela, F., Avlund, K., & Bohr, V. (2013). Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells. Nature Communications, 5(11), 850-864. http://www.impactaging.com/papers/v5/n11/abs/100618a.html

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title = "Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells",

abstract = "Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.",

author = "Scott Maynard and Guido Keijzers and Martin Gram and Claus Desler and Laila Bendix and Esben Budtz-J{\o}rgensen and Drude Molbo and Croteau, {Deborah L} and Merete Osler and Tinna Stevnsner and Rasmussen, {Lene Juel} and Flemming Dela and Kirsten Avlund and Vilhelm Bohr",

year = "2013",

month = nov,

day = "11",

language = "English",

volume = "5",

pages = "850--864",

journal = "Nature Communications",

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Maynard, S, Keijzers, G, Gram, M, Desler, C, Bendix, L, Budtz-Jørgensen, E, Molbo, D, Croteau, DL, Osler, M, Stevnsner, T, Rasmussen, LJ, Dela, F, Avlund, K & Bohr, V 2013, 'Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells', Nature Communications, bind 5, nr. 11, s. 850-864. <http://www.impactaging.com/papers/v5/n11/abs/100618a.html>

Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells. / Maynard, Scott; Keijzers, Guido; Gram, Martin et al.
I: Nature Communications, Bind 5, Nr. 11, 11.11.2013, s. 850-864.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

AU - Maynard, Scott

AU - Keijzers, Guido

AU - Gram, Martin

AU - Desler, Claus

AU - Bendix, Laila

AU - Budtz-Jørgensen, Esben

AU - Molbo, Drude

AU - Croteau, Deborah L

AU - Osler, Merete

AU - Stevnsner, Tinna

AU - Rasmussen, Lene Juel

AU - Dela, Flemming

AU - Avlund, Kirsten

AU - Bohr, Vilhelm

PY - 2013/11/11

Y1 - 2013/11/11

N2 - Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.

AB - Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.

M3 - Journal article

C2 - 24304678

SN - 2041-1723

VL - 5

SP - 850

EP - 864

JO - Nature Communications

JF - Nature Communications

IS - 11

ER -

Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

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