Refined candidate region specified by haplotype sharing for Escherichia coli F4ab/F4ac susceptibility alleles in pigs

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  • M Jacobsen, Danmark
  • S S Kracht, Danmark
  • G Esteso, Genomica y Mejora Animal, Departamento de Genética, Universidad de Córdoba, Spanien
  • S Cirera, Danmark
  • I Edfors, School of Pure and Applied Natural Sciences, University of Kalmar, Sverige
  • A L Archibald, Danmark
  • C Bendixen
  • L Andersson, Danmark
  • M Fredholm, Danmark
  • C B Jørgensen, Danmark
  • Cellevægsbiologi og Molekylær Virologi
  • Molekylær Genetik og Systembiologi
  • Institut for Genetik og Bioteknologi
Infection of the small intestine by enterotoxigenic Escherichia coli F4ab/ac is a major welfare problem and financial burden for the pig industry. Natural resistance to this infection is inherited as a Mendelian recessive trait, and a polymorphism in the MUC4 gene segregating for susceptibility/resistance is presently used in a selection programme by the Danish pig breeding industry. To elucidate the genetic background involved in E. coli F4ab/ac susceptibility in pigs, a detailed haplotype map of the porcine candidate region was established. This region covers approximately 3.7 Mb. The material used for the study is a three generation family, where the founders are two Wild boars and eight Large White sows. All pigs have been phenotyped for susceptibility to F4ab/ac using an adhesion assay. Their haplotypes are known from segregation analysis using flanking markers. By a targeted approach, the candidate region was subjected to screening for polymorphisms, mainly focusing on intronic sequences. A total of 18 genes were partially sequenced, and polymorphisms were identified in GP5, CENTB2, APOD, PCYT1A, OSTalpha, ZDHHC19, TFRC, ACK1, MUC4, MUC20, KIAA0226, LRCH3 and MUC13. Overall, 227 polymorphisms were discovered in the founder generation. The analysis revealed a large haplotype block, spanning at least 1.5 Mb around MUC4, to be associated with F4ab/ac susceptibility
Udgivelsesdato: February
OriginalsprogEngelsk
TidsskriftAnimal Genetics
Vol/bind41
Nummer1
Sider (fra-til)21-25
Antal sider4
ISSN0268-9146
DOI
StatusUdgivet - 2010

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