TY - JOUR
T1 - Reference intervals and stability of haptocorrin and holotranscobalamin in Danish children and elderly
AU - Knudsen, Cindy S.
AU - Parkner, Tina
AU - Hoffmann-Lücke, Elke
AU - Abildgaard, Anders
AU - Greibe, Eva
PY - 2023/6
Y1 - 2023/6
N2 - Background: Haptocorrin (HC) and holotranscobalamin (holoTC) carry vitamin B12 (B12) in the circulation and can be useful biomarkers for evaluating B12 status. The concentration of both proteins depends on age, but data on reference intervals for children and the elderly are sparse. Similarly, not much is known about the effect of preanalytical factors. Methods: HC plasma samples from healthy elderly > 65 years (n = 124) were analysed, and both HC and holoTC were analysed in paediatric serum samples ≤ 18 years (n = 400). Furthermore, we investigated assay precision and stability. Results: HC and holoTC were effected by age. We established reference intervals for HC: 2–10 years, 369–1237 pmol/L; 11–18 years, 314–1128 pmol/L; 65–82 years, 242–680 pmol/L and for holoTC: 2–10 years, 46–206 pmol/L; 11-18 years, 30–178 pmol/L. Analytical coefficients of variations of 6.0–6.8% and 7.9–15.7% were found for HC and holoTC, respectively. HC were affected when stored at room temperature and by freeze/thaw. HoloTC was stable at room temperature and after delayed centrifugation. Conclusion: We present novel 95% age-related reference limits for HC and HoloTC in children, and for HC both in children and elderly. Moreover, we found HoloTC to be fairly stable when stored, whereas HC was more vulnerable to preanalytical factors.
AB - Background: Haptocorrin (HC) and holotranscobalamin (holoTC) carry vitamin B12 (B12) in the circulation and can be useful biomarkers for evaluating B12 status. The concentration of both proteins depends on age, but data on reference intervals for children and the elderly are sparse. Similarly, not much is known about the effect of preanalytical factors. Methods: HC plasma samples from healthy elderly > 65 years (n = 124) were analysed, and both HC and holoTC were analysed in paediatric serum samples ≤ 18 years (n = 400). Furthermore, we investigated assay precision and stability. Results: HC and holoTC were effected by age. We established reference intervals for HC: 2–10 years, 369–1237 pmol/L; 11–18 years, 314–1128 pmol/L; 65–82 years, 242–680 pmol/L and for holoTC: 2–10 years, 46–206 pmol/L; 11-18 years, 30–178 pmol/L. Analytical coefficients of variations of 6.0–6.8% and 7.9–15.7% were found for HC and holoTC, respectively. HC were affected when stored at room temperature and by freeze/thaw. HoloTC was stable at room temperature and after delayed centrifugation. Conclusion: We present novel 95% age-related reference limits for HC and HoloTC in children, and for HC both in children and elderly. Moreover, we found HoloTC to be fairly stable when stored, whereas HC was more vulnerable to preanalytical factors.
KW - Haptocorrin
KW - Holotranscobalamin
KW - Reference interval
KW - Stability study
KW - Validation
KW - Vitamin B12 binding proteins
KW - Transcobalamins/analysis
KW - Humans
KW - Vitamin B 12 Deficiency
KW - Vitamin B 12
KW - Biomarkers
KW - Denmark
KW - Aged
KW - Child
UR - http://www.scopus.com/inward/record.url?scp=85162231190&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2023.117394
DO - 10.1016/j.cca.2023.117394
M3 - Journal article
C2 - 37209861
AN - SCOPUS:85162231190
SN - 0009-8981
VL - 546
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
M1 - 117394
ER -