Reduced heat shock response in human mononuclear cells during aging and its association with polymorphisms in HSP70 genes

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Reduced heat shock response in human mononuclear cells during aging and its association with polymorphisms in HSP70 genes. / Singh, Ripudaman; Kølvraa, Steen; Bross, Peter; Jensen, Uffe Birk; Gregersen, Niels; Tan, Qihua; Knudsen, Christian; Rattan, Suresh I S.

I: Cell Stress & Chaperones, Bind 11, Nr. 3, 01.01.2006, s. 208-215.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Singh, Ripudaman ; Kølvraa, Steen ; Bross, Peter ; Jensen, Uffe Birk ; Gregersen, Niels ; Tan, Qihua ; Knudsen, Christian ; Rattan, Suresh I S. / Reduced heat shock response in human mononuclear cells during aging and its association with polymorphisms in HSP70 genes. I: Cell Stress & Chaperones. 2006 ; Bind 11, Nr. 3. s. 208-215.

Bibtex

@article{2a556ddf45e54c9689d08d62e3815b4b,
title = "Reduced heat shock response in human mononuclear cells during aging and its association with polymorphisms in HSP70 genes",
abstract = "Age-dependent changes in heat shock response (HSR) were studied in mononuclear cells (monocytes and lymphocytes) collected from young (mean age = 22.6 +/- 1.7 years) and middle-aged (mean age = 56.3 +/- 4.7 years) subjects after 1 hour of heat shock at 42 degrees C. Genotype-specific HSR was measured by genotyping the subjects for 3 single nucleotide polymorphisms, HSPA1A(A-110C), HSPA1B(A1267G), and HSPA1L(T2437C), 1 each in the 3 HSP70 genes. A significant age-related decrease in the induction of Hsp70 occurred after heat shock in both monocytes and lymphocytes. The noninducible and inducible forms of Hsp70 decreased 1.3-fold (P <0.001) and 1.4-fold (P <0.001), respectively, in the monocytes with age. In the young subjects, a positive association was found between HSPA1L(T2437C) polymorphism and HSR. CC carriers had a significantly lower induction than TT carriers in both monocytes (P = 0.015) and lymphocytes (P = 0.044). This polymorphism, which is present in the coding region of HSPA1L gene, can affect the chaperoning function of Hsp70. These data consolidate our other observations that the CC genotype is unfavorable for human longevity and provide a functional explanation in terms of variations in HSR.",
keywords = "Adult, Aging, Cells, Cultured, Female, HSP70 Heat-Shock Proteins, Heat-Shock Response, Humans, Leukocytes, Mononuclear, Lymphocytes, Male, Middle Aged, Polymorphism, Genetic, Polymorphism, Single Nucleotide",
author = "Ripudaman Singh and Steen K{\o}lvraa and Peter Bross and Jensen, {Uffe Birk} and Niels Gregersen and Qihua Tan and Christian Knudsen and Rattan, {Suresh I S}",
year = "2006",
month = "1",
day = "1",
language = "English",
volume = "11",
pages = "208--215",
journal = "Cell Stress & Chaperones",
issn = "1355-8145",
publisher = "Springer Netherlands",
number = "3",

}

RIS

TY - JOUR

T1 - Reduced heat shock response in human mononuclear cells during aging and its association with polymorphisms in HSP70 genes

AU - Singh, Ripudaman

AU - Kølvraa, Steen

AU - Bross, Peter

AU - Jensen, Uffe Birk

AU - Gregersen, Niels

AU - Tan, Qihua

AU - Knudsen, Christian

AU - Rattan, Suresh I S

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Age-dependent changes in heat shock response (HSR) were studied in mononuclear cells (monocytes and lymphocytes) collected from young (mean age = 22.6 +/- 1.7 years) and middle-aged (mean age = 56.3 +/- 4.7 years) subjects after 1 hour of heat shock at 42 degrees C. Genotype-specific HSR was measured by genotyping the subjects for 3 single nucleotide polymorphisms, HSPA1A(A-110C), HSPA1B(A1267G), and HSPA1L(T2437C), 1 each in the 3 HSP70 genes. A significant age-related decrease in the induction of Hsp70 occurred after heat shock in both monocytes and lymphocytes. The noninducible and inducible forms of Hsp70 decreased 1.3-fold (P <0.001) and 1.4-fold (P <0.001), respectively, in the monocytes with age. In the young subjects, a positive association was found between HSPA1L(T2437C) polymorphism and HSR. CC carriers had a significantly lower induction than TT carriers in both monocytes (P = 0.015) and lymphocytes (P = 0.044). This polymorphism, which is present in the coding region of HSPA1L gene, can affect the chaperoning function of Hsp70. These data consolidate our other observations that the CC genotype is unfavorable for human longevity and provide a functional explanation in terms of variations in HSR.

AB - Age-dependent changes in heat shock response (HSR) were studied in mononuclear cells (monocytes and lymphocytes) collected from young (mean age = 22.6 +/- 1.7 years) and middle-aged (mean age = 56.3 +/- 4.7 years) subjects after 1 hour of heat shock at 42 degrees C. Genotype-specific HSR was measured by genotyping the subjects for 3 single nucleotide polymorphisms, HSPA1A(A-110C), HSPA1B(A1267G), and HSPA1L(T2437C), 1 each in the 3 HSP70 genes. A significant age-related decrease in the induction of Hsp70 occurred after heat shock in both monocytes and lymphocytes. The noninducible and inducible forms of Hsp70 decreased 1.3-fold (P <0.001) and 1.4-fold (P <0.001), respectively, in the monocytes with age. In the young subjects, a positive association was found between HSPA1L(T2437C) polymorphism and HSR. CC carriers had a significantly lower induction than TT carriers in both monocytes (P = 0.015) and lymphocytes (P = 0.044). This polymorphism, which is present in the coding region of HSPA1L gene, can affect the chaperoning function of Hsp70. These data consolidate our other observations that the CC genotype is unfavorable for human longevity and provide a functional explanation in terms of variations in HSR.

KW - Adult

KW - Aging

KW - Cells, Cultured

KW - Female

KW - HSP70 Heat-Shock Proteins

KW - Heat-Shock Response

KW - Humans

KW - Leukocytes, Mononuclear

KW - Lymphocytes

KW - Male

KW - Middle Aged

KW - Polymorphism, Genetic

KW - Polymorphism, Single Nucleotide

M3 - Journal article

C2 - 17009593

VL - 11

SP - 208

EP - 215

JO - Cell Stress & Chaperones

JF - Cell Stress & Chaperones

SN - 1355-8145

IS - 3

ER -