Redox balance and immunity of piglets pre‑ and post‑E. coli challenge after treatment with hemp or fish oil, and vitamin E

TY - JOUR

T1 - Redox balance and immunity of piglets pre‑ and post‑E. coli challenge after treatment with hemp or fish oil, and vitamin E

AU - Madsen, Pernille Aagaard

AU - Jensen, Søren Krogh

AU - Lauridsen, Charlotte

PY - 2024/5/14

Y1 - 2024/5/14

N2 - This study investigated the influence of polyunsaturated fatty acid composition and vitamin E supplementation on oxidative status and immune responses in weanling piglets pre- and post-E. coli challenge. Suckling piglets (n = 24) were randomly selected from two litters for an oral supplementation (1 mL/day) with fish oil or hemp oil and vitamin E supplementation (60 mg natural vitamin E/mL oil) from day 10 to 28 of age. At day 29 and 30 of age, each piglet was orally inoculated with 6.7 × 108 and 3.96 × 108 CFU of F4 and F18 E. coli, respectively. Blood was sampled from all piglets on day 28 before E. coli challenge and on day 35 of age to investigate immunological and oxidative stress markers in plasma. One week after weaning and exposure to E. coli, a general reduction in the α-tocopherol concentration and activity of GPX1 was obtained. Vitamin E supplementation lowered the extent of lipid peroxidation and improved the antioxidative status and immune responses after E. coli challenge. Hemp oil had the greatest effect on antioxidant enzyme activity. Provision of hemp oil and vitamin E to suckling piglets may reduce the incidence of post-weaning diarrhea.

AB - This study investigated the influence of polyunsaturated fatty acid composition and vitamin E supplementation on oxidative status and immune responses in weanling piglets pre- and post-E. coli challenge. Suckling piglets (n = 24) were randomly selected from two litters for an oral supplementation (1 mL/day) with fish oil or hemp oil and vitamin E supplementation (60 mg natural vitamin E/mL oil) from day 10 to 28 of age. At day 29 and 30 of age, each piglet was orally inoculated with 6.7 × 108 and 3.96 × 108 CFU of F4 and F18 E. coli, respectively. Blood was sampled from all piglets on day 28 before E. coli challenge and on day 35 of age to investigate immunological and oxidative stress markers in plasma. One week after weaning and exposure to E. coli, a general reduction in the α-tocopherol concentration and activity of GPX1 was obtained. Vitamin E supplementation lowered the extent of lipid peroxidation and improved the antioxidative status and immune responses after E. coli challenge. Hemp oil had the greatest effect on antioxidant enzyme activity. Provision of hemp oil and vitamin E to suckling piglets may reduce the incidence of post-weaning diarrhea.

KW - antioxidants

KW - biomarkers

KW - enteric infection

KW - fatty acid composition

KW - oxidative stress

KW - pigs

KW - Antioxidants

KW - Biomarkers

KW - Enteric infection

KW - Fatty acid composition

KW - Oxidative stress

KW - Pigs

UR - http://www.scopus.com/inward/record.url?scp=85193207293&partnerID=8YFLogxK

U2 - 10.1038/s41598-024-61927-1

DO - 10.1038/s41598-024-61927-1

M3 - Journal article

C2 - 38744900

SN - 2045-2322

VL - 14

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 11053

ER -