Reconstitution of Na,K-ATPase in Nanodiscs

Jonas Lindholt Gregersen, Natalya Fedosova, Poul Nissen, Thomas Boesen

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Abstract

Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows studies of the membrane proteins in detergent-free aqueous solutions in a lipid bilayer. Here, we apply the technique to the Na +,K +-ATPase (NKA) from pig kidney using Membrane Scaffolding Protein 1 D1 (MSP1D1). Contrary to other reports, the nanodiscs obtained by our protocol are built up of the native lipids originally present in the detergent solubilized sample together with the NKA.

OriginalsprogEngelsk
TidsskriftMethods in Molecular Biology
Vol/bind1377
Sider (fra-til)403-409
Antal sider7
StatusUdgivet - 1 jan. 2016

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