Abstract
Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows studies of the membrane proteins in detergent-free aqueous solutions in a lipid bilayer. Here, we apply the technique to the Na +,K +-ATPase (NKA) from pig kidney using Membrane Scaffolding Protein 1 D1 (MSP1D1). Contrary to other reports, the nanodiscs obtained by our protocol are built up of the native lipids originally present in the detergent solubilized sample together with the NKA.
Originalsprog | Engelsk |
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Tidsskrift | Methods in Molecular Biology |
Vol/bind | 1377 |
Sider (fra-til) | 403-409 |
Antal sider | 7 |
Status | Udgivet - 1 jan. 2016 |