Recombinase-independent AAV for anterograde transsynaptic tracing

Islam Faress; Valentina Khalil; Haruka Yamamoto; Szilard Sajgo; Keisuke Yonehara; Sadegh Nabavi

doi:10.1186/s13041-023-01053-7

Recombinase-independent AAV for anterograde transsynaptic tracing

Islam Faress^*, Valentina Khalil, Haruka Yamamoto, Szilard Sajgo, Keisuke Yonehara, Sadegh Nabavi

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

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Abstract

Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging.

Originalsprog	Engelsk
Artikelnummer	66
Tidsskrift	Molecular Brain
Vol/bind	16
Nummer	1
DOI	https://doi.org/10.1186/s13041-023-01053-7
Status	Udgivet - sep. 2023

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10.1186/s13041-023-01053-7Licens: CC BY

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title = "Recombinase-independent AAV for anterograde transsynaptic tracing",

abstract = "Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging.",

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author = "Islam Faress and Valentina Khalil and Haruka Yamamoto and Szilard Sajgo and Keisuke Yonehara and Sadegh Nabavi",

note = "Publisher Copyright: {\textcopyright} 2023, Min Zhuo, Bong-Kiun Kaang and BioMed central Ltd.",

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AU - Faress, Islam

AU - Khalil, Valentina

AU - Yamamoto, Haruka

AU - Sajgo, Szilard

AU - Yonehara, Keisuke

AU - Nabavi, Sadegh

PY - 2023/9

Y1 - 2023/9

N2 - Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging.

AB - Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging.

KW - AAV

KW - Neural-circuit

KW - Neuroanatomy

KW - Transsynaptic

KW - Viral-tracing

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AN - SCOPUS:85171345292

SN - 1756-6606

VL - 16

JO - Molecular Brain

JF - Molecular Brain

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ER -

Recombinase-independent AAV for anterograde transsynaptic tracing

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