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Recombinant adeno-associated virus mediated gene delivery in the extracranial nervous system of adult mice by direct nerve immersion

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

DOI

This protocol outlines a minimally invasive and quickly performed approach for transgene delivery in the extracranial nervous system of adult mice using recombinant adeno-associated virus (AAV). The technique, named Sciatic Nerve Direct Immersion (SciNDi), relies on the direct bilateral immersion of the exposed sciatic nerve with AAV. We show that in comparison with intramuscular AAV delivery, SciNDi results in widespread transduction in connected neuroanatomical tracts both in the sciatic nerve trunk and the lumbar spinal cord. For complete details on the use and execution of this protocol, please refer to Jan et al. (2019) and Richner et al. (2011, 2017).

OriginalsprogEngelsk
Artikelnummer101181
TidsskriftSTAR Protocols
Vol/bind3
Nummer1
Antal sider15
DOI
StatusUdgivet - mar. 2022

Bibliografisk note

Funding Information:
This work was supported by funding to A.J. in the form of a Marie Skłodowska Curie Fellowship from the European Union’s Horizon 2020 Research and Innovation Programme ( MSCA -IF-2017, grant #786433 ) and Lundbeckfonden , Denmark (grant #R250-2017-1131 ). M.R. was funded by Lundbeckfonden, Denmark (grant #R313-2019-606 ) and J.N.R. was supported by the Villum Foundation and Henny Sophie Clausen og møbelarkitekt Aksel Clausens Fond . The authors would like to thank Helene Andersen (JRN lab) and Sandra Bonnesen (CV lab) for technical help during the study.

Funding Information:
This work was supported by funding to A.J. in the form of a Marie Sk?odowska Curie Fellowship from the European Union's Horizon 2020 Research and Innovation Programme (MSCA-IF-2017, grant #786433) and Lundbeckfonden, Denmark (grant #R250-2017-1131). M.R. was funded by Lundbeckfonden, Denmark (grant #R313-2019-606) and J.N.R. was supported by the Villum Foundation and Henny Sophie Clausen og m?belarkitekt Aksel Clausens Fond. The authors would like to thank Helene Andersen (JRN lab) and Sandra Bonnesen (CV lab) for technical help during the study. M.R. and A.J. designed and performed research; C.B.V. P.H.J. and J.R.N. contributed with infrastructural support and data evaluation; M.R. N.P.G. and A.J. wrote the manuscript; M.R. and A.J. drafted the graphical abstract using BioRender, under license to C.V. (co-author). All authors read and approved the manuscript. The authors declare no competing interests.

Publisher Copyright:
© 2022 The Author(s)

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