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Ramularia collo-cygni effectors and their role in planta

Publikation: Bog/antologi/afhandling/rapportPh.d.-afhandlingForskning

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Ramularia collo-cygni effectors and their role in planta. / Lopez, Jean-Baptiste.

2018. 181 s.

Publikation: Bog/antologi/afhandling/rapportPh.d.-afhandlingForskning

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Lopez, Jean-Baptiste. / Ramularia collo-cygni effectors and their role in planta. 2018. 181 s.

Bibtex

@phdthesis{98ef4eb15fe54b0fb174fc7334475ee3,
title = "Ramularia collo-cygni effectors and their role in planta",
abstract = "Ramullaria collo-cygni (Rcc) is an apoplasmic fungus that hasbeen characterized recently as an emerging barley pathogen. Rcc is thecausal agent of the Ramularia leaf spot disease, responsible forsignificant damage on barley crops worldwide. Rcc has beenpreviously studied from the developmental and epidemiologic pointof view, but few studies have been done at the molecular level.During my PhD, I studied this interaction with a focus on theeffectors proteins. Effectors are small secreted protein allowing microorganismto bypass or counteract plant immune defense. In order tobe able to study those proteins, I first developed a pipeline allowing ina semi high-throughput fashion cloning expression and screening inthe host plant of the effectors.In the second part of this thesis I report my work on thefunctional characterization of a novel effector produced via thepreviously mentioned pipeline.Finally, I contributed to a joint project on comparativetranscriptomic between 2 Rcc isolates infecting 2 barley cultivars withcontrasting sensitivities to Rcc. My first focus was to understand howRcc adapt its transcriptome depending on the host. Secondly, I wasinterested to look at the differential regulation between both isolatestranscriptomes while infecting barley.",
keywords = "ramularia collo-cygni, effectors, screening method, chitin binding, comparative transcriptomic",
author = "Jean-Baptiste Lopez",
note = "Udgivet: 20-11-2017",
year = "2018",
language = "English",

}

RIS

TY - BOOK

T1 - Ramularia collo-cygni effectors and their role in planta

AU - Lopez, Jean-Baptiste

N1 - Udgivet: 20-11-2017

PY - 2018

Y1 - 2018

N2 - Ramullaria collo-cygni (Rcc) is an apoplasmic fungus that hasbeen characterized recently as an emerging barley pathogen. Rcc is thecausal agent of the Ramularia leaf spot disease, responsible forsignificant damage on barley crops worldwide. Rcc has beenpreviously studied from the developmental and epidemiologic pointof view, but few studies have been done at the molecular level.During my PhD, I studied this interaction with a focus on theeffectors proteins. Effectors are small secreted protein allowing microorganismto bypass or counteract plant immune defense. In order tobe able to study those proteins, I first developed a pipeline allowing ina semi high-throughput fashion cloning expression and screening inthe host plant of the effectors.In the second part of this thesis I report my work on thefunctional characterization of a novel effector produced via thepreviously mentioned pipeline.Finally, I contributed to a joint project on comparativetranscriptomic between 2 Rcc isolates infecting 2 barley cultivars withcontrasting sensitivities to Rcc. My first focus was to understand howRcc adapt its transcriptome depending on the host. Secondly, I wasinterested to look at the differential regulation between both isolatestranscriptomes while infecting barley.

AB - Ramullaria collo-cygni (Rcc) is an apoplasmic fungus that hasbeen characterized recently as an emerging barley pathogen. Rcc is thecausal agent of the Ramularia leaf spot disease, responsible forsignificant damage on barley crops worldwide. Rcc has beenpreviously studied from the developmental and epidemiologic pointof view, but few studies have been done at the molecular level.During my PhD, I studied this interaction with a focus on theeffectors proteins. Effectors are small secreted protein allowing microorganismto bypass or counteract plant immune defense. In order tobe able to study those proteins, I first developed a pipeline allowing ina semi high-throughput fashion cloning expression and screening inthe host plant of the effectors.In the second part of this thesis I report my work on thefunctional characterization of a novel effector produced via thepreviously mentioned pipeline.Finally, I contributed to a joint project on comparativetranscriptomic between 2 Rcc isolates infecting 2 barley cultivars withcontrasting sensitivities to Rcc. My first focus was to understand howRcc adapt its transcriptome depending on the host. Secondly, I wasinterested to look at the differential regulation between both isolatestranscriptomes while infecting barley.

KW - ramularia collo-cygni

KW - effectors

KW - screening method

KW - chitin binding

KW - comparative transcriptomic

M3 - Ph.D. thesis

BT - Ramularia collo-cygni effectors and their role in planta

ER -