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Ramularia collo-cygni effectors and their role in planta

Publikation: Bog/antologi/afhandling/rapportPh.d.-afhandling

  • Jean-Baptiste Lopez
Ramullaria collo-cygni (Rcc) is an apoplasmic fungus that has
been characterized recently as an emerging barley pathogen. Rcc is the
causal agent of the Ramularia leaf spot disease, responsible for
significant damage on barley crops worldwide. Rcc has been
previously studied from the developmental and epidemiologic point
of view, but few studies have been done at the molecular level.

During my PhD, I studied this interaction with a focus on the
effectors proteins. Effectors are small secreted protein allowing microorganism
to bypass or counteract plant immune defense. In order to
be able to study those proteins, I first developed a pipeline allowing in
a semi high-throughput fashion cloning expression and screening in
the host plant of the effectors.
In the second part of this thesis I report my work on the
functional characterization of a novel effector produced via the
previously mentioned pipeline.

Finally, I contributed to a joint project on comparative
transcriptomic between 2 Rcc isolates infecting 2 barley cultivars with
contrasting sensitivities to Rcc. My first focus was to understand how
Rcc adapt its transcriptome depending on the host. Secondly, I was
interested to look at the differential regulation between both isolates
transcriptomes while infecting barley.
OriginalsprogEngelsk
Antal sider181
StatusUdgivet - 2018

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