Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System

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Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System. / Sultan, Abida; Andersen, Birgit; Christensen, Jesper Bjerg; Poulsen, Hanne Damgaard; Svensson, Birte; Finnie, Christine.

I: Journal of Agricultural and Food Chemistry, Bind 67, Nr. 22, 06.2019, s. 6432-6444.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Sultan, A, Andersen, B, Christensen, JB, Poulsen, HD, Svensson, B & Finnie, C 2019, 'Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System', Journal of Agricultural and Food Chemistry, bind 67, nr. 22, s. 6432-6444. https://doi.org/10.1021/acs.jafc.9b01708

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Author

Sultan, Abida ; Andersen, Birgit ; Christensen, Jesper Bjerg ; Poulsen, Hanne Damgaard ; Svensson, Birte ; Finnie, Christine. / Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System. I: Journal of Agricultural and Food Chemistry. 2019 ; Bind 67, Nr. 22. s. 6432-6444.

Bibtex

@article{155b2ef9e4464317be63f163c8cf9e04,
title = "Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System",
abstract = "Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.",
keywords = "barley proteome, mass spectrometry, NADPH-dependent thioredoxin reductase/thioredoxin system, protease inhibitors",
author = "Abida Sultan and Birgit Andersen and Christensen, {Jesper Bjerg} and Poulsen, {Hanne Damgaard} and Birte Svensson and Christine Finnie",
year = "2019",
month = "6",
doi = "10.1021/acs.jafc.9b01708",
language = "English",
volume = "67",
pages = "6432--6444",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "AMER CHEMICAL SOC",
number = "22",

}

RIS

TY - JOUR

T1 - Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System

AU - Sultan, Abida

AU - Andersen, Birgit

AU - Christensen, Jesper Bjerg

AU - Poulsen, Hanne Damgaard

AU - Svensson, Birte

AU - Finnie, Christine

PY - 2019/6

Y1 - 2019/6

N2 - Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.

AB - Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx). Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis. Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.

KW - barley proteome

KW - mass spectrometry

KW - NADPH-dependent thioredoxin reductase/thioredoxin system

KW - protease inhibitors

UR - http://www.scopus.com/inward/record.url?scp=85066436943&partnerID=8YFLogxK

U2 - 10.1021/acs.jafc.9b01708

DO - 10.1021/acs.jafc.9b01708

M3 - Journal article

C2 - 31095381

AN - SCOPUS:85066436943

VL - 67

SP - 6432

EP - 6444

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 22

ER -