Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO

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DOI

  • Stine Gry Kristensen, Rigshospitalet
  • ,
  • Ajay Kumar, Ansh Laboratories LLC
  • ,
  • Bhanu Kalra, Ansh Laboratories LLC
  • ,
  • Susanne Elisabeth Pors, Rigshospitalet
  • ,
  • Jane Alrø Bøtkjær, Rigshospitalet
  • ,
  • Linn Salto Mamsen, Rigshospitalet
  • ,
  • Lotte Berdiin Colmorn, Rigshospitalet
  • ,
  • Jens Fedder
  • Erik Ernst
  • Lisa Ann Owens, Imperial College London
  • ,
  • Kate Hardy, Imperial College London
  • ,
  • Stephen Franks, Imperial College London
  • ,
  • Claus Yding Andersen, Laboratory of Reproductive, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Københavns Universitet

CONTEXT: Members of the TGF-β family have been implicated in aberrant follicle development in women with polycystic ovaries (PCO). OBJECTIVE: Are there quantitative differences in the concentrations of TGF-β family members in fluid from human small antral follicles (hSAFs) in women with or without PCO? DESIGN AND SETTING: Follicle fluids (FFs) were collected from 4- to 11-mm hSAFs obtained from women undergoing ovarian tissue cryopreservation for fertility preservation. PATIENTS: FFs from 16 women with PCO (FF = 93) and 33 women without PCO (FF = 92). MAIN OUTCOME MEASURES: Intrafollicular concentrations of growth differentiation factor-9 (GDF9); anti-Müllerian hormone (AMH); inhibin-A and inhibin-B; total inhibin; activin-A, activin-B, and activin-AB; follistatin; follistatin-like-3; estradiol; and testosterone. RESULTS: Activin-B concentrations were reported in hSAFs, and concentrations were 10 times higher than activin-A and activin-AB concentrations. Activin-B showed significant associations with other growth factors. Concentrations of inhibin-A and inhibin-B were significantly lower in FFs from women with PCO, especially in hSAFs <8 mm in diameter. AMH concentrations did not differ between the groups in hSAFs <8 mm; however, AMH remained high in hSAFs >8 mm in women with PCO but decreased in women without PCO. Estradiol was significantly lower in FFs from women with PCO and showed significant associations with AMH. Concentrations of GDF9 showed significantly higher concentrations in PCO FFs of follicles >6 mm. CONCLUSIONS: Altered concentrations of TGF-β family members in hSAFs from women with PCO highlight altered growth factor signaling as a potential mechanism for follicle growth arrest.

OriginalsprogEngelsk
TidsskriftThe Journal of clinical endocrinology and metabolism
Vol/bind104
Nummer12
Sider (fra-til)6371-6384
Antal sider14
ISSN0021-972X
DOI
StatusUdgivet - 2019

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