Abstract
Background:
Kidney transplantation is the preferred treatment of choice for selected patients with end-stage kidney disease (ESKD). Unfortunately, there is an imbalance between organ supply and organ demand, which could be reduced by accepting more marginal kidneys. There is therefore a need of improved preservation methods to deal with the increasing number of marginal donor kidneys, and in parallel, a need to introduce new diagnostic measures that allow quantitative information about the viability and microcirculation/oxygenation in the kidney.
Laser speckle contrast imaging (LSCI), fluorescence based imaging (FBI) and optical coherence tomography (OCT) are optical imaging technologies, providing images that reflect microperfusion, microvascularisation and microanatomy. Due to their high spatial resolution and non-invasive performance (FBI is minimally invasive, due to the demand of contrast agents), we believe that these imaging technologies are promising methods for evaluating kidney viability prior to transplantation. Besides, information about such intrarenal microcirculatory markers could become beneficial for evaluating potential marginal donor kidneys in future.
The aim of this study was to evaluate the feasibility of LSCI, FBI and OCT for assessment of kidney microperfusion and microvascularisation.
Methods:
Porcine kidneys are withdrawn from healthy pigs and connected to an ex vivo machine perfusion system with blood as an oxygen carrier. LSCI, FBI, and OCT systems were then placed close to the kidney. All systems are able to perform imaging in the renal cortex. The renal cortex’ microperfusion is gradually altered during the experimental procedure by introducing an ex vivo embolization model based on microsphere infusion in the arterial side of the ex vivo perfusion system. By positioning a reference tissue perfusion- and oxygenation sensor in the kidney parenchyma during the imaging procedures, we can compare our optical imaging-based (LSCI, FBI, and OCT) measurements with measurements obtained from reference invasive techniques.
Altogether, by performing LSCI, FBI, and OCT on ex vivo perfused kidneys, we seek to quantify kidney microperfusion and microvascularisation and validate these measurements against an accepted reference method: an invasive microprobe (Oxford Optronics perfusion and oxygenation sensors).
Kidney transplantation is the preferred treatment of choice for selected patients with end-stage kidney disease (ESKD). Unfortunately, there is an imbalance between organ supply and organ demand, which could be reduced by accepting more marginal kidneys. There is therefore a need of improved preservation methods to deal with the increasing number of marginal donor kidneys, and in parallel, a need to introduce new diagnostic measures that allow quantitative information about the viability and microcirculation/oxygenation in the kidney.
Laser speckle contrast imaging (LSCI), fluorescence based imaging (FBI) and optical coherence tomography (OCT) are optical imaging technologies, providing images that reflect microperfusion, microvascularisation and microanatomy. Due to their high spatial resolution and non-invasive performance (FBI is minimally invasive, due to the demand of contrast agents), we believe that these imaging technologies are promising methods for evaluating kidney viability prior to transplantation. Besides, information about such intrarenal microcirculatory markers could become beneficial for evaluating potential marginal donor kidneys in future.
The aim of this study was to evaluate the feasibility of LSCI, FBI and OCT for assessment of kidney microperfusion and microvascularisation.
Methods:
Porcine kidneys are withdrawn from healthy pigs and connected to an ex vivo machine perfusion system with blood as an oxygen carrier. LSCI, FBI, and OCT systems were then placed close to the kidney. All systems are able to perform imaging in the renal cortex. The renal cortex’ microperfusion is gradually altered during the experimental procedure by introducing an ex vivo embolization model based on microsphere infusion in the arterial side of the ex vivo perfusion system. By positioning a reference tissue perfusion- and oxygenation sensor in the kidney parenchyma during the imaging procedures, we can compare our optical imaging-based (LSCI, FBI, and OCT) measurements with measurements obtained from reference invasive techniques.
Altogether, by performing LSCI, FBI, and OCT on ex vivo perfused kidneys, we seek to quantify kidney microperfusion and microvascularisation and validate these measurements against an accepted reference method: an invasive microprobe (Oxford Optronics perfusion and oxygenation sensors).
| Originalsprog | Engelsk |
|---|---|
| Publikationsdato | 24 nov. 2022 |
| Status | Udgivet - 24 nov. 2022 |
| Begivenhed | 5th Annual Research Meeting, Institute for Clinical Medicine, Aarhus University - Aarhus University Hospital, Aarhus, Danmark Varighed: 24 nov. 2022 → 24 nov. 2022 |
Konference
| Konference | 5th Annual Research Meeting, Institute for Clinical Medicine, Aarhus University |
|---|---|
| Lokation | Aarhus University Hospital |
| Land/Område | Danmark |
| By | Aarhus |
| Periode | 24/11/2022 → 24/11/2022 |