Purification of human complement protein c5

Lars Sottrup-Jensen, Gregers Rom Andersen

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    Abstract

    Complement C5 is cleaved by proteolysis in the terminal phase of complement activation generating the pro-inflammatory C5a and membrane attack complex nucleator C5b. Whereas purification of its paralogues C3 and C4 from plasma is relatively straightforward, C5 purification is more complicated due to the lower amounts present and overlaps with the much more abundant C3 during several chromatographic steps. Here we describe our procedure for purifying homogenous, monodisperse, and crystallizable C5.
    OriginalsprogEngelsk
    TitelThe Complete System : Mehods and Protocols
    Antal sider10
    Vol/bind1100
    ForlagSpringer
    Publikationsdato2014
    Sider93-102
    ISBN (Trykt)978-1-62703-723-5
    ISBN (Elektronisk)978-1-62703-724-2
    DOI
    StatusUdgivet - 2014
    NavnMethods in Molecular Biology
    ISSN1064-3745

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