Protocol for generating customizable and reproducible plots of sequencing coverage data using the seqNdisplayR package

Søren Lykke-Andersen*, Jérôme O. Rouvière, Manfred Schmid, Maria Gockert, Torben Heick Jensen*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Abstract

The widespread usage of next-generation sequencing methods for functional genomics studies requires standardized tools for consistent visualization of the associated data. Here, we present seqNdisplayR, an R package for plotting standard sequencing data coverage within a genomic region of interest in a customizable and reproducible manner. We describe steps for installing software, preparing data files, choosing options, and plotting data. This tool is readily available for users with no prior experience with R through the “Shiny app” interface. For complete details on the use and execution of this protocol, please refer to Lykke-Andersen et al.,1 Gockert et al.,2 and Rouviere et al.3

OriginalsprogEngelsk
Artikelnummer102960
TidsskriftSTAR Protocols
Vol/bind5
Nummer2
DOI
StatusUdgivet - jun. 2024

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