Proteome reference maps of the Lotus japonicus nodule and root

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Proteome reference maps of the Lotus japonicus nodule and root. / Dam, Svend; Dyrlund, Thomas Franck; Ussatjuk, Anna; Jochimsen, Bjarne; Nielsen, Kasper; Goffard, Nicolas; Ventosa, Miguel André Lorenzo; Lorentzen, Andrea Maria; Gupta, Vikas; Andersen, Stig U; Enghild, Jan J; Ronson, Clive W; Roepstorff, Peter; Stougaard, Jens.

I: Proteomics, Bind 14, Nr. 2-3, 02.2014, s. 230-240.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Dam, S, Dyrlund, TF, Ussatjuk, A, Jochimsen, B, Nielsen, K, Goffard, N, Ventosa, MAL, Lorentzen, AM, Gupta, V, Andersen, SU, Enghild, JJ, Ronson, CW, Roepstorff, P & Stougaard, J 2014, 'Proteome reference maps of the Lotus japonicus nodule and root', Proteomics, bind 14, nr. 2-3, s. 230-240. https://doi.org/10.1002/pmic.201300353

APA

Dam, S., Dyrlund, T. F., Ussatjuk, A., Jochimsen, B., Nielsen, K., Goffard, N., Ventosa, M. A. L., Lorentzen, A. M., Gupta, V., Andersen, S. U., Enghild, J. J., Ronson, C. W., Roepstorff, P., & Stougaard, J. (2014). Proteome reference maps of the Lotus japonicus nodule and root. Proteomics, 14(2-3), 230-240. https://doi.org/10.1002/pmic.201300353

CBE

Dam S, Dyrlund TF, Ussatjuk A, Jochimsen B, Nielsen K, Goffard N, Ventosa MAL, Lorentzen AM, Gupta V, Andersen SU, Enghild JJ, Ronson CW, Roepstorff P, Stougaard J. 2014. Proteome reference maps of the Lotus japonicus nodule and root. Proteomics. 14(2-3):230-240. https://doi.org/10.1002/pmic.201300353

MLA

Vancouver

Dam S, Dyrlund TF, Ussatjuk A, Jochimsen B, Nielsen K, Goffard N o.a. Proteome reference maps of the Lotus japonicus nodule and root. Proteomics. 2014 feb;14(2-3):230-240. https://doi.org/10.1002/pmic.201300353

Author

Dam, Svend ; Dyrlund, Thomas Franck ; Ussatjuk, Anna ; Jochimsen, Bjarne ; Nielsen, Kasper ; Goffard, Nicolas ; Ventosa, Miguel André Lorenzo ; Lorentzen, Andrea Maria ; Gupta, Vikas ; Andersen, Stig U ; Enghild, Jan J ; Ronson, Clive W ; Roepstorff, Peter ; Stougaard, Jens. / Proteome reference maps of the Lotus japonicus nodule and root. I: Proteomics. 2014 ; Bind 14, Nr. 2-3. s. 230-240.

Bibtex

@article{6304d6ed651f479bab0f969e48c339ae,
title = "Proteome reference maps of the Lotus japonicus nodule and root",
abstract = "Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high throughput transcriptomics. We have employed proteomics to further understand nodule development. Two developmental stages representing nodules prior to nitrogen fixation (white) and mature nitrogen fixing nodules (red) were compared with roots. In addition, the proteome of a spontaneous nodule formation mutant (snf1) was determined. From nodules and roots, 780 and 790 protein spots from 2D gels were identified and approximately 45% of the corresponding unique gene accessions were common. Including a previous proteomics set from Lotus pod and seed, the common gene accessions were decreased to 7%. Interestingly, an indication of more pronounced post translational modifications in nodules than in roots was determined. Between the two nodule developmental stages, higher levels of pathogen related 10 proteins, HSP's, and proteins involved in redox processes were found in white nodules, suggesting a higher stress level at this developmental stage. In contrast, protein spots corresponding to nodulins such as leghemoglobin, asparagine synthetase, sucrose synthase, and glutamine synthetase were prevalent in red nodules. The distinct biochemical state of nodules was further highlighted by the conspicuous presence of several nitrilases, ascorbate metabolic enzymes and putative rhizobial effectors.",
keywords = "comparative analysis, Lotus japonicus, proteomics, root, root nodule",
author = "Svend Dam and Dyrlund, {Thomas Franck} and Anna Ussatjuk and Bjarne Jochimsen and Kasper Nielsen and Nicolas Goffard and Ventosa, {Miguel Andr{\'e} Lorenzo} and Lorentzen, {Andrea Maria} and Vikas Gupta and Andersen, {Stig U} and Enghild, {Jan J} and Ronson, {Clive W} and Peter Roepstorff and Jens Stougaard",
note = ";",
year = "2014",
month = feb,
doi = "10.1002/pmic.201300353",
language = "English",
volume = "14",
pages = "230--240",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "2-3",

}

RIS

TY - JOUR

T1 - Proteome reference maps of the Lotus japonicus nodule and root

AU - Dam, Svend

AU - Dyrlund, Thomas Franck

AU - Ussatjuk, Anna

AU - Jochimsen, Bjarne

AU - Nielsen, Kasper

AU - Goffard, Nicolas

AU - Ventosa, Miguel André Lorenzo

AU - Lorentzen, Andrea Maria

AU - Gupta, Vikas

AU - Andersen, Stig U

AU - Enghild, Jan J

AU - Ronson, Clive W

AU - Roepstorff, Peter

AU - Stougaard, Jens

N1 - ;

PY - 2014/2

Y1 - 2014/2

N2 - Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high throughput transcriptomics. We have employed proteomics to further understand nodule development. Two developmental stages representing nodules prior to nitrogen fixation (white) and mature nitrogen fixing nodules (red) were compared with roots. In addition, the proteome of a spontaneous nodule formation mutant (snf1) was determined. From nodules and roots, 780 and 790 protein spots from 2D gels were identified and approximately 45% of the corresponding unique gene accessions were common. Including a previous proteomics set from Lotus pod and seed, the common gene accessions were decreased to 7%. Interestingly, an indication of more pronounced post translational modifications in nodules than in roots was determined. Between the two nodule developmental stages, higher levels of pathogen related 10 proteins, HSP's, and proteins involved in redox processes were found in white nodules, suggesting a higher stress level at this developmental stage. In contrast, protein spots corresponding to nodulins such as leghemoglobin, asparagine synthetase, sucrose synthase, and glutamine synthetase were prevalent in red nodules. The distinct biochemical state of nodules was further highlighted by the conspicuous presence of several nitrilases, ascorbate metabolic enzymes and putative rhizobial effectors.

AB - Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high throughput transcriptomics. We have employed proteomics to further understand nodule development. Two developmental stages representing nodules prior to nitrogen fixation (white) and mature nitrogen fixing nodules (red) were compared with roots. In addition, the proteome of a spontaneous nodule formation mutant (snf1) was determined. From nodules and roots, 780 and 790 protein spots from 2D gels were identified and approximately 45% of the corresponding unique gene accessions were common. Including a previous proteomics set from Lotus pod and seed, the common gene accessions were decreased to 7%. Interestingly, an indication of more pronounced post translational modifications in nodules than in roots was determined. Between the two nodule developmental stages, higher levels of pathogen related 10 proteins, HSP's, and proteins involved in redox processes were found in white nodules, suggesting a higher stress level at this developmental stage. In contrast, protein spots corresponding to nodulins such as leghemoglobin, asparagine synthetase, sucrose synthase, and glutamine synthetase were prevalent in red nodules. The distinct biochemical state of nodules was further highlighted by the conspicuous presence of several nitrilases, ascorbate metabolic enzymes and putative rhizobial effectors.

KW - comparative analysis

KW - Lotus japonicus

KW - proteomics

KW - root

KW - root nodule

U2 - 10.1002/pmic.201300353

DO - 10.1002/pmic.201300353

M3 - Journal article

C2 - 24293220

VL - 14

SP - 230

EP - 240

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 2-3

ER -