Aarhus University Seal / Aarhus Universitets segl

Proteome analysis of pod and seed development in the model legume Lotus japonicus

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Gitte Nautrup-Pedersen, Danmark
  • Svend Dam, Danmark
  • Brian S Laursen, Danmark
  • Astrid L Siegumfeldt
  • ,
  • Kasper Nielsen
  • ,
  • Nicolas Goffard
  • ,
  • Hans Henrik Stærfeldt
  • ,
  • Carsten Friis
  • ,
  • Shusei Sato
  • ,
  • Satoshi Tabata
  • ,
  • Andrea Lorentzen
  • ,
  • Peter Roepstorff
  • ,
  • Jens Stougaard
  • Molekylærbiologisk Institut
Legume pods serve important functions during seed development and are themselves sources of food and feed. Compared to seeds, the metabolism and development of pods are not well-defined. The present characterization of pods from the model legume Lotus japonicus, together with the detailed analyses of the pod and seed proteomes in five developmental stages, paves the way for comparative pathway analysis and provides new metabolic information. Proteins were analyzed by two-dimensional gel electrophoresis and tandem-mass spectrometry. These analyses lead to the identification of 604 pod proteins and 965 seed proteins, including 263 proteins distinguishing the pod. The complete data set is publicly available at http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi , where spots in a reference map are linked to experimental data, such as matched peptides, quantification values, and gene accessions. Identified pod proteins represented enzymes from 85 different metabolic pathways, including storage globulins and a late embryogenesis abundant protein. In contrast to seed maturation, pod maturation was associated with decreasing total protein content, especially proteins involved in protein biosynthesis and photosynthesis. Proteins detected only in pods included three enzymes participating in the urea cycle and four in nitrogen and amino group metabolism, highlighting the importance of nitrogen metabolism during pod development. Additionally, five legume seed proteins previously unassigned in the glutamate metabolism pathway were identified.
OriginalsprogEngelsk
TidsskriftJournal of Proteome Research
Vol/bind9
Nummer11
Sider (fra-til)5715-26
Antal sider12
ISSN1535-3893
DOI
StatusUdgivet - 5 nov. 2010

Se relationer på Aarhus Universitet Citationsformater

ID: 34383517