TY - JOUR
T1 - Protein-Templated Reactions Using DNA-Antibody Conjugates
AU - Pellejero, Lorena Baranda
AU - Nijenhuis, Minke A. D.
AU - Ricci, Francesco
AU - Gothelf, Kurt
PY - 2023/3/29
Y1 - 2023/3/29
N2 - DNA-templated chemical reactions have found wide applications in drug discovery, programmed multistep synthesis, nucleic acid detection, and targeted drug delivery. The control of these reactions has, however, been limited to nucleic acid hybridization as a means to direct the proximity between reactants. In this work a system capable of translating protein-protein binding events into a DNA-templated reaction which leads to the covalent formation of a product is introduced. Protein-templated reactions by employing two DNA-antibody conjugates that are both able to recognize the same target protein and to colocalize a pair of reactant DNA strands able to undergo a click reaction are achieved. Two individual systems, each responsive to human serum albumin (HSA) and human IgG, are engineered and it is demonstrated that, while no reaction occurs in the absence of proteins, both protein-templated reactions can occur simultaneously in the same solution without any inter-system crosstalk.
AB - DNA-templated chemical reactions have found wide applications in drug discovery, programmed multistep synthesis, nucleic acid detection, and targeted drug delivery. The control of these reactions has, however, been limited to nucleic acid hybridization as a means to direct the proximity between reactants. In this work a system capable of translating protein-protein binding events into a DNA-templated reaction which leads to the covalent formation of a product is introduced. Protein-templated reactions by employing two DNA-antibody conjugates that are both able to recognize the same target protein and to colocalize a pair of reactant DNA strands able to undergo a click reaction are achieved. Two individual systems, each responsive to human serum albumin (HSA) and human IgG, are engineered and it is demonstrated that, while no reaction occurs in the absence of proteins, both protein-templated reactions can occur simultaneously in the same solution without any inter-system crosstalk.
KW - bioconjugation
KW - DNA templated reactions
KW - nanotechnology
KW - proteins
KW - proximity effect
KW - MULTISTEP ORGANIC-SYNTHESIS
KW - NANOSTRUCTURES
KW - LIGATION
KW - BINDING
KW - RNA
UR - http://www.scopus.com/inward/record.url?scp=85127226698&partnerID=8YFLogxK
U2 - 10.1002/smll.202200971
DO - 10.1002/smll.202200971
M3 - Journal article
C2 - 35344264
SN - 1613-6810
VL - 19
JO - Small
JF - Small
IS - 13
M1 - 2200971
ER -