Protein binding assays for an accurate differentiation of vitamin B12 from its inactive analogue. A study on edible cricket powder

TY - JOUR

T1 - Protein binding assays for an accurate differentiation of vitamin B12 from its inactive analogue. A study on edible cricket powder

AU - Fedosov, Sergey

AU - Nexø, Ebba

AU - Würtz Heegaard, Christian

AU - Goldin , Jarrod

AU - Manson, Joel B.

PY - 2023/10/30

Y1 - 2023/10/30

N2 - Inactive analogues of vitamin B12 (cobalamin, Cbl) can mimic the active Cbl in food if using the traditional microbiological measurements. Thus, overestimated Cbl was recently revealed in edible insects employing immunoaffinity adsorption, HPLC-separation and mass spectrometry (https://doi.org/10.1016/j.foodchem.2021.129048). Here we demonstrate the utility of a convenient binding assay to evaluate Cbl in edible cricket powders. The assay employed the Cbl-specific protein intrinsic factor (IF) and the analogue-detecting protein haptocorrin. The excessive analogues had a weak affinity for IF, resulting in a modest overestimate of Cbl. This overestimate was corrected by a novel mathematical procedure, based on the ratio of analogue/Cbl in the sample and their relative affinities for IF. We found that 100 g of cricket powders contained 40–60 µg of analogues and 0.75–2.2 μg of Cbl. This result was confirmed by HPLC. A correct approach to Cbl-measurements is essential for nutritional assessment of any analogue-containing food.

AB - Inactive analogues of vitamin B12 (cobalamin, Cbl) can mimic the active Cbl in food if using the traditional microbiological measurements. Thus, overestimated Cbl was recently revealed in edible insects employing immunoaffinity adsorption, HPLC-separation and mass spectrometry (https://doi.org/10.1016/j.foodchem.2021.129048). Here we demonstrate the utility of a convenient binding assay to evaluate Cbl in edible cricket powders. The assay employed the Cbl-specific protein intrinsic factor (IF) and the analogue-detecting protein haptocorrin. The excessive analogues had a weak affinity for IF, resulting in a modest overestimate of Cbl. This overestimate was corrected by a novel mathematical procedure, based on the ratio of analogue/Cbl in the sample and their relative affinities for IF. We found that 100 g of cricket powders contained 40–60 µg of analogues and 0.75–2.2 μg of Cbl. This result was confirmed by HPLC. A correct approach to Cbl-measurements is essential for nutritional assessment of any analogue-containing food.

KW - Correction

KW - Corrinoids

KW - Cricket powder

KW - Haptocorrin

KW - Intrinsic factor

KW - Measurement

KW - Vitamin B12

UR - http://www.scopus.com/inward/record.url?scp=85167508746&partnerID=8YFLogxK

U2 - 10.1016/j.fochx.2023.100824

DO - 10.1016/j.fochx.2023.100824

M3 - Journal article

C2 - 37780289

SN - 2590-1575

VL - 19

JO - Food Chemistry: X

JF - Food Chemistry: X

M1 - 100824

ER -