Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR

Stine Kristensen; Gitte Albinus Pedersen; Lene Niemann Nejsum; Duncan S Sutherland

doi:10.1021/jp4038528

Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR

Stine Kristensen, Gitte Albinus Pedersen, Lene Niemann Nejsum, Duncan S Sutherland

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

13 Citationer (Scopus)

Abstract

This paper presents the use of the quartz microbalance with dissipation combined with surface plasmon resonance to probe protein adsorption at nanopatterned surfaces. Three different types of adsorbing materials, representing rigid discrete nanoparticles, dense protein films and soft low density films have been studied on systematic varied circular nanostructures in the 100 nm to 1000 nm size range. Analysis and quantification of the QCM-D response from larger nanostructures could be understood and quantified in the same way as for homogeneous surfaces, while that for nanostructures of 100 nm and 200 nm diameter was significantly underestimated. Our findings suggest a size limitation of those techniques in analysis of adsorption at nanofeatures.

Originalsprog	Engelsk
Tidsskrift	Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical
Vol/bind	117
Nummer	36
Sider (fra-til)	10376-10383
Antal sider	8
ISSN	1520-6106
DOI	https://doi.org/10.1021/jp4038528
Status	Udgivet - 12 sep. 2013

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10.1021/jp4038528

Citationsformater

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title = "Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR",

abstract = "This paper presents the use of the quartz microbalance with dissipation combined with surface plasmon resonance to probe protein adsorption at nanopatterned surfaces. Three different types of adsorbing materials, representing rigid discrete nanoparticles, dense protein films and soft low density films have been studied on systematic varied circular nanostructures in the 100 nm to 1000 nm size range. Analysis and quantification of the QCM-D response from larger nanostructures could be understood and quantified in the same way as for homogeneous surfaces, while that for nanostructures of 100 nm and 200 nm diameter was significantly underestimated. Our findings suggest a size limitation of those techniques in analysis of adsorption at nanofeatures. ",

author = "Stine Kristensen and Pedersen, {Gitte Albinus} and Nejsum, {Lene Niemann} and Sutherland, {Duncan S}",

year = "2013",

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doi = "10.1021/jp4038528",

language = "English",

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Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR. / Kristensen, Stine; Pedersen, Gitte Albinus ; Nejsum, Lene Niemann et al.
I: Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical, Bind 117, Nr. 36, 12.09.2013, s. 10376-10383.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR

AU - Kristensen, Stine

AU - Pedersen, Gitte Albinus

AU - Nejsum, Lene Niemann

AU - Sutherland, Duncan S

PY - 2013/9/12

Y1 - 2013/9/12

N2 - This paper presents the use of the quartz microbalance with dissipation combined with surface plasmon resonance to probe protein adsorption at nanopatterned surfaces. Three different types of adsorbing materials, representing rigid discrete nanoparticles, dense protein films and soft low density films have been studied on systematic varied circular nanostructures in the 100 nm to 1000 nm size range. Analysis and quantification of the QCM-D response from larger nanostructures could be understood and quantified in the same way as for homogeneous surfaces, while that for nanostructures of 100 nm and 200 nm diameter was significantly underestimated. Our findings suggest a size limitation of those techniques in analysis of adsorption at nanofeatures.

AB - This paper presents the use of the quartz microbalance with dissipation combined with surface plasmon resonance to probe protein adsorption at nanopatterned surfaces. Three different types of adsorbing materials, representing rigid discrete nanoparticles, dense protein films and soft low density films have been studied on systematic varied circular nanostructures in the 100 nm to 1000 nm size range. Analysis and quantification of the QCM-D response from larger nanostructures could be understood and quantified in the same way as for homogeneous surfaces, while that for nanostructures of 100 nm and 200 nm diameter was significantly underestimated. Our findings suggest a size limitation of those techniques in analysis of adsorption at nanofeatures.

U2 - 10.1021/jp4038528

DO - 10.1021/jp4038528

M3 - Journal article

C2 - 23863103

SN - 1520-6106

VL - 117

SP - 10376

EP - 10383

JO - Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical

JF - Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical

IS - 36

ER -

Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR

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