Prevention of Initial Bacterial Attachment by Osteopontin and Other Bioactive Milk Proteins

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6 Citationer (Scopus)

Abstract

A considerable body of work has studied the involvement of osteopontin (OPN) in human physiology and pathology, but comparably little is known about the interaction of OPN with prokaryotic cells. Recently, bovine milk OPN has been proposed as a therapeutic agent to prevent the build-up of dental biofilms, which are responsible for the development of caries lesions. Bioactive milk proteins are among the most exciting resources for caries control, as they hamper bacterial attachment to teeth without affecting microbial homeostasis in the mouth. The present work investigated the ability of OPN to prevent the adhesion of three dental biofilm-forming bacteria to saliva-coated surfaces under shear-controlled flow conditions in comparison with the major milk proteins alpha-lactalbumin, beta-lactoglobulin, alpha s1-casein, beta-casein and kappa-casein, as well as crude milk protein. OPN was the most effective single protein to reduce the adhesion of Actinomyces naeslundii, Lactobacillus paracasei subsp. paracasei and Streptococcus mitis. beta-casein and crude milk protein also had a pronounced effect on all three species, which suggests binding to different microbial surface structures rather than the blocking of a specific bacterial adhesin. Bioactive milk proteins show potential to delay harmful biofilm formation on teeth and hence the onset of biofilm-related oral disease.

OriginalsprogEngelsk
Artikelnummer1922
TidsskriftBiomedicines
Vol/bind10
Nummer8
Antal sider8
ISSN2227-9059
DOI
StatusUdgivet - aug. 2022

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