Practical considerations for rapid and quantitative NMR-based metabolomics

Frans A.A. Mulder*, Leonardo Tenori, Cristina Licari, Claudio Luchinat*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

7 Citationer (Scopus)

Abstract

NMR is a key technology for metabolomics because of its robustness and reproducibility. Herein we discuss practical considerations that extend the utility of NMR spectroscopy. First, the long T1 spin relaxation times of small molecules limits high-throughput data acquisition because most experimental time is lost while waiting for signal recovery. In principle, the addition of a small amount of commercially-available paramagnetic gadolinium chelate allows cost-effective and efficient high-throughput mixture analysis with correct concentration determination. However, idle time caused by slow temperature regulation during sample exchanges, poses a next constraint. We show how, with proper care, NMR sample scanning times can be reduced additionally by a factor of two. Lastly, we describe how equidistant bucketing is a simple and fast procedure for metabolomic fingerprinting. The combination of these advancements help to make NMR metabolomics more versatile than it is today.

OriginalsprogEngelsk
Artikelnummer107462
TidsskriftJournal of Magnetic Resonance
Vol/bind352
Antal sider10
ISSN1090-7807
DOI
StatusUdgivet - jul. 2023
Udgivet eksterntJa

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